DIFFERENTIATION OF HUMAN HEPATOCARCINOMA SMMC-7721 CELLS INDUCED BY HMBA
- 生命科学－已发表论文 
本文研究HMBA对人肝癌SMMC-7721细胞的诱导分化作用。细胞生长曲线测定和细胞分裂指数观察显示HMBA可明显抑制细胞增殖,细胞生长抑制率达64.14%,分裂指数抑制率为53.88%。光镜和透射电镜观察可见HMBA能诱导人肝癌SMMC-7721细胞形态和超微结构发生恢复性改变。生化检测或免疫细胞化学方法观察显示,HMBA处理后细胞γ-谷氨酸转肽酶(γ-GT)活性和甲胎蛋白(AFP)、增殖细胞核抗原(PCNA)表达均降低,而酪氨酸-酮戊二酸转氨酶(TAT)活性增强。流式细胞仪分析表明HMBA引起细胞发生G_0/G_1期阻滞。以上结果表明HMBA能有效抑制人肝癌细胞恶性增殖活性,逆转肝癌细胞恶性形态与超微结构特征,改变肝癌细胞相关酶活性和抗原表达,引发G_0/G_1期阻滞,从而对肝癌细胞具有明显的诱导分化作用。In this paper, the effects of HMBA on the differentiation of human hepatocarcinoma cell line SMMC-7721 were investigated. After treated with 5mmol/L HMBA, the proliferation of SMMC-7721 cells was inhibited remarkably, the cell growth inhibitory rate amounted to 64.14%, the cell mitotic index was declined by 53.88 % . Light microscopy and transmission electron microscopy showed that the morphology and ultrastructure of the cells treated with HMBA undergone restorational alteration. Cytochemistry and immunocytochemistry assay revealed that the activities of 7-GT declined and the levels of AFP and PCNA downregulated while the activity of TAT increased significantly after HMBA treatment. In the meantime, flow cytometry analysis showed that HMBA could arrest the cells in G0/G1 phase. The results showed that HMBA could effectively inhibit the proliferation, reverse the malignant morphology and ultrastructure, alter the levels of enzymes and antigens, arrest the cells in G0/G1 , and induce the differentiation of human hepatocarcinoma SMMC-7721 cells in vitro.