Studies on tissue specificity of LDH isozyme in Sparus latus
以黄鳍鲷为材料进行两方面研究 :( 1 )用聚丙烯酰胺凝胶电泳方法 ,对其肌肉、心脏、肝脏、血清、玻璃体、晶状体、脑等组织器官进行LDH同工酶分析比较 .结果表明 ,不同组织中LDH的同工酶谱带不相同 ,存在着组织特异性 ;( 2 )用聚丙烯酰胺等电聚焦电泳法对其肌肉、血清的LDH同工酶进行分离 ,结果得到 1 2～ 1 5条电泳区带 ,等电点范围在 pH为 3.6 5～ 9.58之间This experiment was done on Sparus latus in two aspects: (1)Lactate dehydrogenase(LDH) isozymes of muscle, heart, liver, serum, crystalline len, vitreum and brain were analyzed by means of polyacrylamide gel disc electrophoresis (PAGE). Two bands were given in the electrophorogram of heart, liver and crystalline len respectively; four were given in that of vitreum while specificity in Sparus latus . The results show that the gene of LDH expresses best in muscle, then in heart, liver, serum. (2)The LDH isozymes of muscle and serum were further isolated by using disc isoelectric focusing in polyacrylamide gel. There were more than ten subbands, and their isoelectric points were in the range of 3.65～9.58.