The Inhibition Effect of Tachyplesin on the Proliferation of Human Gastric Carcinoma Cell Line BGC-823
- 生命科学－已发表论文 
应用从中国鲎血细胞中提取的鲎素处理人胃癌 BGC- 82 3细胞 ,研究海洋生物活性物质的抗肿瘤作用 .实验结果显示 ,经 2 .0 μg/m L鲎素处理的 BGC- 82 3细胞生长缓慢 ,倍增时间延长 ,细胞集落形成能力减弱 ,细胞生长抑制率达 62 .67% ,细胞分裂指数下降 4 0 .91% .处理后细胞碱性磷酸酶细胞化学反应减弱、分布改变 ,碱性磷酸酶和乳酸脱氢酶活力分别下降 4 0 .59%和 76.0 5% .结果表明 ,鲎素能有效地抑制胃癌细胞的增殖活动 ,具有与癌细胞诱导分化物相似的抗肿瘤效果 .Tachyplesin, which was isolated from acid extracts of horseshoe crab ( Tachypleus tridentatus ) hemocytes, was used to treat the human gastric carcinoma cell line BGC 823 and to investigate the antitumor effects of marine biological active substances. It was found that the BGC 823 Cells, after treated with 2.0 μg/mL tachyplesin, grew slowly, the doubling time enlonged, the colony formation ability decreased, the growth inhibitory rate amounted to 62.67%, and cellular mitotic index decreased by 40.91%. In the meantime, tachyplesin could change the distribution of AKPase and decline the abilities of AKPase by 40.59% and LDH 76.05% respectively. These results indicated that tachyplesin could effectively inhibite the proliferation of the human gastric adenocarcinoma cell line BGC 823 and act as a differentiational inducer in its antitumor effects.