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dc.contributor.author杨善民
dc.date.accessioned2017-11-14T02:46:09Z
dc.date.available2017-11-14T02:46:09Z
dc.date.issued2000-05-15
dc.identifier.citation细胞生物学杂志,2000,(02):25-28
dc.identifier.issn0253-9977
dc.identifier.otherXBZZ200002005
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/154094
dc.description.abstract为探索自发性高血压大鼠动脉平滑肌细胞(SMC)增生的机理,采用~3H-TdR标记、端粒酶活性以及细胞周期蛋白D_1基因RT-PCR检测分别对10周龄SHR、WKY大动脉及其体外分离的SMC进行研究。成年、高血压状态的SHR胸、腹主动脉段端粒酶有高的活性,而同龄、同源WKY大鼠者则没有。从成年SHR腹主动脉段分离的SMC~3H—TdR的掺入率比WKY者约提高了43%。成年高血压状态下的SHR腹主动脉SMC细胞周期蛋白D_1基因的RT-PCR的产物与WKY者相差不明显。
dc.description.abstractTo understand the mechanism of proliferation of smooth muscle cells (SMC) in spontaneously hypertensive rat (SHR) ,the aortae and the SMC seperated from aortae in ten week old SHR were studied by the assay of telomerase, incorperation of 3H-TdR and cyclin Dt RT-PCR,in contrast to the age-matched normotensive WKY. The telomerase activity of thoracic and abdominal aortae in adult SHR is reactivated,but is not any in that of WKY. Smooth muscle cells isolated from the aortae in the adult SHR expressed telomerase activity,but little,if any,in that of WKY. In vitro, incorperation of 3H-TdR in SMC from the abdominal in adult SHR is higher (43%)than that of WKY. The gene product of Cyclin D, amplified by RT-PCR from the SMC of hypertensive SHR abdominal aorta is no particular difference from that of WKY.
dc.description.sponsorship教育部细胞生物学和肿瘤细胞工程重点实验室开放研究经费部分资助
dc.language.isozh_CN
dc.subjectSIR
dc.subject动脉平滑肌细胞
dc.subject端粒酶细胞周期蛋白D
dc.subjectRT-PCR
dc.subjectAortal smooth muscle cells Telomerase Cyclin D1 RT-PCR
dc.title成年SHR动脉平滑肌细胞端粒酶活性和周期蛋白D_1的研究
dc.title.alternativeTELOMERASE ACTIVITY AND CYCLIN D_1 OF SMOOTH MUSCLE CELLS FROM AORTAE IN THE ADULT SHR
dc.typeArticle


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