A Study on Embryo Membrane of Horseshoe Crab (Tachypleus tridentatus) Using Scanning Microscopy
- 生命科学－已发表论文 
中国鲎的胚胎主要有三层被膜 :卵壳膜、内卵膜和胚护膜 .它们对胚胎发育的正常进行至关重要 .扫描电镜研究结果显示 ,卵壳膜被覆于成熟的卵子和 S19之前的胚胎表面 ,厚约 80 .0 μm,是中国鲎卵子和早期胚胎的主要保护性结构 ;内卵膜在晚期囊胚和原肠胚期形成 ,至胚胎发育完成时蜕去 ,厚约 18.0～ 32 .0 μm,外表面布满大小不等、结构相似的凸起的结构单位 ,内表面衬有直径约0 .1μm,互联呈网的纤维 ,内卵膜和在 S19时才完全形成的胚护膜是晚期胚胎的主要保护性结构 .Embryo membranes are very important for developing horseshoe crab embryo. In the present study, the embryo membrane changes of horseshoe crab(Tachypleus tridentatus)with the development of embryo were observed. Chorion, which formed in the egg development, was covered in the surface of embryo before S19 embryo and about 80.0 μm in thickness. The inner membrane shaped in later blastula and early gastrulae was 18.0~32.0 μm thick and became thinner and thinner with growth of embryo until it was discarded by first year instar. The outer surface of inner membrane was constituted by many similar protruding unites while the inner surface was covered by fibers which were 0.1 μm in diameter and connected each other. The embryo membrane appearing at last stage was the embryonic cuticle, formed in S11 to S19 stage and disappeared together with inner membrane when embryo development finished.