In Vitro Selection And Physiological And Biochemical Analyses of Salt-Tolerant Variant For Wheat
- 生命科学－已发表论文 
以小麦幼穗、幼胚为原始材料，进行耐盐突变体的筛选，并对其愈伤组织及再生植株后代进行耐盐稳定性的生理生化分析．结果表明：（１）耐盐系在高盐浓度下其鲜重、干重明显高于对照系；（２）耐盐系保持较高的Ｋ＋ ／Ｎａ＋ 比；（３）对照系种子有１１ 条醇溶蛋白电泳带，而耐盐系为１４ 条，与对照系相比Ｂ２、Ｂ３、Ｂ４ 带为耐盐所特有，Ｂ１ 带含量高于对照物，但Ｂ５ 带含量低于对照系；（４）耐盐系再生植株后代可溶蛋白电泳带为２８ 条，而对照系为２６ 条蛋白带，９８ ｋＤ、７５ ｋＤ、５２ ｋＤ、４９ ｋＤ、３２ ｋＤ为耐盐系所特有蛋白带，３８ ｋＤ、３５ ｋＤ和２１ ｋＤ蛋白带为对照系所特有．并对小麦耐盐突变体筛选相关问题进行讨论．The immature embryo and young inflorescence segments of wheat( Triticum aestivum )were used for selecting salt tolerant variant.The genetic stability of callus and their progenies were studied through physiological and biochemical analyses. The results showed that (1) The salt tolerant callus maintained more fresh weight and dry weight even at higher NaCl concerntration than the control callus;(2)The salt tolerant callus maintained higher K +/Na + ratio; (3)Three additional bands of gliadin(B2,B3,B4) were found in the grains of salt tolerant wheat line and B1 band content increased.However B5 band of the control was markedly stronger than that in salt tolerant line; and 4.28 bands of soluble protein appeared in seedling of salt tolerant variant, whereas only 26 bands in CK. 98 kD,75 kD,52 kD,49 kD and 32 kD bands were specific in salt tolerant line, 38 kD,35 kD and 21 kD protein bands only appeared in the control. Characters of salt tolerance in wheat were discussed.