Analysis of Epistatic and QE Interaction Effects of QTL Controlling Heading Date in Rice (Oryza sativa L.)
- 生命科学－已发表论文 
抽穗期是水稻的重要农艺性状,深入了解其遗传效应对水稻育种实践具有重要现实意义。本研究利用基于明恢86×佳辐占、广陆矮×佳辐占两个重组自交系构建的SSR遗传图谱,应用混合线性模型方法对2003年晚季和2005年早季获得的两季水稻抽穗期数据进行QTL定位,并作加性效应、加性×加性上位互作效应及环境互作效应分析。两个群体共检测到10个控制抽穗期的QTL,分别位于1、2、3、6、7和10号染色体上,仅qHD10(广佳重组自交系中为qHD10-1)在两个群体中同时检测到,另检测到11对具有上位效应的互作位点,其中有5个是加性效应显著的QTL。环境互作检测中,发现明佳重组自交系的qHD10和广佳重组自交系的qHD7与环境存在显著互作,贡献率分别为0.34%和2.32%。本研究表明:两群体的抽穗期性状的遗传受环境因素影响较小,特别是明佳组合,较适合作为分子辅助育种的研究材料。Heading date (HD) is an important agronomic trait in rice, and detailed understanding of HD's genetic effects is significant for rice breeding practice. Quantitative Trait Locus (QTL) controlling HD of rice in different seasons and environments was analyzed using two Recombination Inbred Lines (RIL) respectively derived from crosses of Minghui86 × Jiafuzhan (MJ) and Guangluai × Jiafuzhan (GJ) by mixed linear model approach. 10 QTL with additive effects for HD were mapped in the chromosome 1, 2, 3, 6, 7 and 10 totally, and the qHD10 (qHD10-1 as in GJ-RIL) was mapped in both RIL. 22 QTL with epistatic effects for HD in these two RIL were detected, and 5 of them were QTL with additive effects. QTL× environment interaction was detected on qHD10 in MJ and qHD7 in GJ, each explain the phenotypic variation of HD with 0.34% and 2.32%. This demonstrates that QTL× environment interaction has minor influence to HD in these two RIL, and they are suitable for Molecular marker-assisted breeding, specially to MJ population.