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dc.contributor.authorZhu, Zhi
dc.contributor.authorJenkins, Gareth
dc.contributor.authorZhang, Wenhua
dc.contributor.authorZhang, Mingxia
dc.contributor.authorGuan, Zhichao
dc.contributor.authorYang, Chaoyong James
dc.contributor.author杨朝勇
dc.date.accessioned2013-03-26T07:12:27Z
dc.date.available2013-03-26T07:12:27Z
dc.date.issued2012-06
dc.identifier.citationANALYTICAL AND BIOANALYTICAL CHEMISTRY,2012,403(8):2127-2143zh_CN
dc.identifier.issn1618-2642
dc.identifier.urihttp://dx.doi.org/10.1007/s00216-012-5914-x
dc.identifier.uriWOS:000305127200007
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/15358
dc.description.abstractNational Basic Research Program of China [2010CB732402]; National Scientific Foundation of China [21075104, 20805038]; Natural Science Foundation of Fujian Province for Distinguished Young Scholars [2010 J06004]zh_CN
dc.language.isoenzh_CN
dc.publisherSPRINGER HEIDELBERGzh_CN
dc.subjectSingle-molecule amplificationzh_CN
dc.subjectEmulsion PCRzh_CN
dc.subjectMicrofluidic dropletszh_CN
dc.subjectHigh-throughput screeningzh_CN
dc.subjectNext generation DNA sequencingzh_CN
dc.subjectRare mutation detectionzh_CN
dc.subjectThe application of microfluidic droplet PCR for single-molecule amplification and analysis has recently been extensively studied. Microfluidic droplet technology has the advantages of compartmentalizing reactions into discrete volumes, performing highly parallel reactions in monodisperse droplets, reducing cross-contamination between droplets, eliminating PCR bias and nonspecific amplification, as well as enabling fast amplification with rapid thermocycling. Here, we have reviewed the important technical breakthroughs of microfluidic droplet PCR in the past five years and their applications to single-molecule amplification and analysis, such as high-throughput screening, next generation DNA sequencing, and quantitative detection of rare mutations. Although the utilization of microfluidic droplet single-molecule PCR is still in the early stages, its great potential has already been demonstrated and will provide novel solutions to today's biomedical engineering challenges in single-molecule amplification and analysis.zh_CN
dc.titleSingle-molecule emulsion PCR in microfluidic dropletszh_CN
dc.typeArticlezh_CN


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