Show simple item record

dc.contributor.authorZeng, Fanwei
dc.contributor.authorNi, Erru
dc.contributor.authorWang, Shengyu
dc.contributor.authorLv, Sha
dc.contributor.authorCao, Chang
dc.contributor.authorZhang, Haipeng
dc.contributor.authorLv, Haiyan
dc.contributor.authorChen, Xiaoli
dc.contributor.authorYan, Jianghua
dc.contributor.authorLuo, Fanghong
dc.contributor.author罗芳洪
dc.date.accessioned2013-03-25T07:49:35Z
dc.date.available2013-03-25T07:49:35Z
dc.date.issued2012-06
dc.identifier.citationHYBRIDOMA,2012,31(3):209-213zh_CN
dc.identifier.issn1554-0014
dc.identifier.urihttp://dx.doi.org/10.1089/hyb.2011.0115
dc.identifier.uriWOS:000305947600010
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/15344
dc.description.abstractProtein tyrosine phosphatase 1B (PTP1B), a member of the protein tyrosine phosphatase (PTP) family, plays a crucial role in metabolic signaling, with insulin and leptin signaling being well studied. New evidence indicates that PTP1B is also involved in cancer. In the present study, we report on the establishment of a monoclonal antibody specific for catalytic domain of PTP1B (PTP1Bc) generated through the hybridoma method. The monoclonal antibody is measured to have a titer of 4.1 x 10(6) against PTP1Bc in indirect ELISA. Western blot and immunofluorescent analyses indicated that this antibody can specifically combine native PTP1B in MDA-MB-231 and MDA-MB-453 cells. This monoclonal antibody against PTP1Bc can help enhance the understanding of PTP1B-related physiological and pathological mechanisms and may act as a therapeutic agent for diabetes, obesity, and cancer in the future.zh_CN
dc.description.sponsorshipNational Natural Science Foundation of China [30973485, 81172970]; Science and Technology Foundation of Fujian Province of China [2011R1039-1]zh_CN
dc.language.isoenzh_CN
dc.publisherMARY ANN LIEBERT INCzh_CN
dc.subjectTYROSINE-PHOSPHATASE 1Bzh_CN
dc.subjectOVEREXPRESSIONzh_CN
dc.subjectOBESITYzh_CN
dc.titleA Monoclonal Antibody Against the Catalytic Domain of PTP1Bzh_CN
dc.typeArticlezh_CN


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record