Fabrication of chitosan/hydroxylapatite composite rods with a layer-by-layer structure for fracture fixation
Date
2012-07Author
Pu, Xi-Ming
Sun, Zhen-Zhen
Hou, Zhen-Qing
Yang, Yun
Yao, Qing-Qing
Zhang, Qi-Qing
张其清
Collections
- 医学院-已发表论文 [2663]
Abstract
A composite rod for fracture fixation using chitosan (CHI)/hydroxylapatite (HA) was prepared by means of in situ precipitation, which had a layer-by-layer structure, good mechanical properties, and cell compatibilities. The CHI/HA composite rods were precipitated from the chitosan solution with calcium and phosphorus precursors, followed by treatment with a tripolyphosphatetrisodium phosphate solution (pH >13) to crosslink the CHI and to hydrolyze the calcium phosphates to nanocrystalline HA. The results of FTIR, XRD, and TEM measurements confirmed that HA had been formed within the CHI matrix. The effects of the CHI/HA ratios (20/0, 20/1, 20/2, 20/4, and 20/5, w/w) on the mechanical properties were investigated. At the CHI/HA ratio of 20/4 (w/w), the bending strength and modulus of the rods were 133 MPa and 6.8 GPa, respectively. Pre-osteoblast MC3T3-E1 cells were cultured in an extract of the CHI/HA rods (20/4, w/w) to study the cell compatibilities of the composite. The observations indicated that the CHI/HA composite could promote the growth of MC3T3-E1 cells better than the composite without HA (p < 0.05). Furthermore, the co-cultivation of the cells and the CHI/HA composite showed that cells fully spread on the surface of the composite with an obvious cytoskeleton organization, which also revealed that the CHI/HA composite had a good biocompatibility. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 100B: 11791189, 2012.
Citation
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,2012,100B(5):1179-1189URI
http://dx.doi.org/10.1002/jbm.b.31961WOS:000304864500001
https://dspace.xmu.edu.cn/handle/2288/15172