A novel recombinant bioadhesive designed from the non-repeating region of Perna viridis foot protein-1
- 医学院－已发表论文 
The repeating decapeptides of the mussel adhesive protein (MAP) were considered as the basis for mussel adhesion in wet environment for it contains L-3, 4-dihydroxyphenylalanine (DOPA). However, DOPA residue in the Perna viridis foot protein-1 (Pvfp-1) do not exist in the repeating decapeptide sequence but present in the non-repeating region. Therefore, it is quite necessary to evaluate the adhesive capacity of the repeating and non-repeating regions of Pvfp-1. In this study, the sequence of eight repeating decapeptides (R-240) and the sequence of the non-repeating region (C-237) of Pvfp-1 was amplified and expressed, respectively. With adsorption, adhesion and coating analysis, it was found that the recombinant C-237 has comparable adhesion and coating ability compared with that of Cell-Tak (TM) (the positive control) and it was also much better than that of R-240, especially on the non-adhesive PTFE surface. Moreover. C-237 exhibited no cytotoxicity and showed better cell adhesion and spreading abilities than that of R-240 on both glass and PTFE surfaces. Therefore, the recombinant C-237 could be used as bioadhesive for medical purpose and be potentially used as an improver for bio-inert materials when applied in biomedical areas. (C) 2012 Elsevier B.V. All rights reserved.
CitationMATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS，2012,32（5）：1280-1287