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dc.contributor.authorHuang, Yong
dc.contributor.authorZou, Quan
dc.contributor.author邹权
dc.contributor.authorSong, Fei
dc.contributor.authorWang, Xin
dc.contributor.authorShen, Xing Jia
dc.date.accessioned2013-03-15T01:22:52Z
dc.date.available2013-03-15T01:22:52Z
dc.date.issued2012-07
dc.identifier.citationRUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY,2012,38(4):417-421zh_CN
dc.identifier.issn1068-1620
dc.identifier.urihttp://dx.doi.org/10.1134/S1068162012030168
dc.identifier.uriWOS:000306432300010
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/15136
dc.description.abstractMicroRNAs (miRNAs) are an abundant class of approximately 22-nucleotide (nt)-short noncoding RNA molecules present in the genomes of all multicellular organisms that act through base pairing to partially complementary sequences of the 3'untranslated region (UTR) of targeted mRNAs. Using bioinformatic approach, we found that the 3' UTR of the Fibroin L chain (Fib-L) mRNA matches perfectly the nucleotides 2-8 at the 5' end of the miRNA-965 and miRNA-1926. These two miRNAs might act as regulators of Fib-L gene expression at the post-transcriptional level. To examine whether Fib-L is directly targeted by miRNA-965 and miRNA-1926 in vitro, miRNA expression vectors and target reporter vector with 3'UTR of Fib-L were constructed respectively. Two vectors were co similar to transfected into Sf21 cells. The luciferase assay showed that miRNA-965 and miRNA-1926 may down regulate the expression of Fib-L via complementary interaction with the target sites in 3'UTR.zh_CN
dc.description.sponsorshipNatural Science Foundation of China [31172266]; Doctoral Science Foundation of He Nan University of Science [09001578]zh_CN
dc.language.isoenzh_CN
dc.publisherMAIK NAUKA/INTERPERIODICA/SPRINGERzh_CN
dc.subjectsilkwormzh_CN
dc.subjectmiRNAszh_CN
dc.subjecttargetszh_CN
dc.subjectfunctional identificationzh_CN
dc.titleThe regulation of silkworm fibroin L chain production by miRNA-965 and miRNA-1926 in insect cellszh_CN
dc.typeArticlezh_CN


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