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dc.contributor.authorNiu, Xubo
dc.contributor.authorGao, Chuan
dc.contributor.authorLo, Li Jan
dc.contributor.authorLuo, Yue
dc.contributor.authorMeng, Chunmei
dc.contributor.authorHong, Jian
dc.contributor.authorHong, Wanjin
dc.contributor.author洪万进
dc.contributor.authorPeng, Jinrong
dc.date.accessioned2013-03-11T00:47:40Z
dc.date.available2013-03-11T00:47:40Z
dc.date.issued2012-07-15
dc.identifier.citationDEVELOPMENTAL BIOLOGY,2012,367(2):197-207zh_CN
dc.identifier.issn0012-1606
dc.identifier.urihttp://dx.doi.org/10.1016/j.ydbio.2012.05.004
dc.identifier.uriWOS:000305818300010
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/15094
dc.description.abstractThe Sec13-Sec31 heterotetramer serves as the outer coat in the COPII complex, which mediates protein trafficking from the endoplasmic reticulum (ER) to the Golgi apparatus. Although it has been studied in depth in yeast and cultured cells, the role of COPII in organogenesis in a multicellular organism has not. We report here that a zebrafish sec13(sq198) mutant, which exhibits a phenotype of hypoplastic digestive organs, has a mutation in the sec13 gene. The mutant gene encodes a carboxyl-terminus-truncated Sec13 that loses its affinity to Sec31a, which leads to disintegration of the ER structure in various differentiated cells in sec13(sq198), including chondrocytes, intestinal epithelial cells and hepatocytes. Disruption of the ER structure activates an unfolded protein response that eventually causes the cells to undergo cell-cycle arrest and cell apoptosis, which arrest the growth of developing digestive organs in the mutant. Our data provide the first direct genetic evidence that COPII function is essential for the organogenesis of the digestive system. (C) 2012 Elsevier Inc. All rights reserved.zh_CN
dc.description.sponsorshipNational Natural Science Foundation of China [30825025]; "973 Program" [2012CB944500]; Agency for Science, Technology and Research in Singaporezh_CN
dc.language.isoenzh_CN
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEzh_CN
dc.subjectSec13zh_CN
dc.subjectCOPII complexzh_CN
dc.subjectDigestive organszh_CN
dc.subjectLiver developmentzh_CN
dc.subjectZebrafishzh_CN
dc.titleSec13 safeguards the integrity of the endoplasmic reticulum and organogenesis of the digestive system in zebrafishzh_CN
dc.typeArticlezh_CN


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