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dc.contributor.author蔡邦平(厦门市园林植物园)
dc.contributor.author陈俊愉(北京林业大学园林学院)
dc.contributor.author张启翔(北京林业大学园林学院)
dc.contributor.author郭良栋(中国科学院微生物研究所)
dc.contributor.author黄耀坚
dc.contributor.author王增福
dc.date.accessioned2013-03-08T09:14:48Z
dc.date.available2013-03-08T09:14:48Z
dc.date.issued2012-02
dc.identifier.citation北京林业大学学报,2012,34增刊1:82-87zh_CN
dc.identifier.issn1000-1522
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/15088
dc.description第一作者: 蔡邦平,博士,副研究员。主要研究方向: 园林植物与观赏园艺、植物菌根。电话: 0592--2039576 Email : cbangping@ 163.com 地址: 361003 福建省厦门市思明区虎园路25 号厦门市园林植物园。zh_CN
dc.description.abstract[中文文摘]为了解决梅根系共生的丛枝菌根(AM)真菌难以应用形态学鉴定的问题,以巢式PCR的AFLP方法研究梅根系AM真菌DNA多态性。试验采集梅花期的根系样品,应用改良CTAB法提取总DNA,经纯化处理后,应用巢式PCR扩增根系AM真菌基因片段,进行AFLP分析。结果表明,18个梅品种的30个根系样品中,仅有8个样品经巢式PCR后获得纯化的DNA片段,占试验样品数的26.7%;8个样品共得到指纹图谱带24条,各样品平均多态性位点数为3.0个,Nei’s基因多样性为0.4097±0.0848,Shannon信息指数为0.5968±0.0955;利用Nei’s遗传相似性系数聚类,梅品种根系内AMF基因组DNA的聚类类别与梅"品种群"这一分类级别无相关性。该试验为植物根系共生AM真菌DNA多态性研究提供了一种简便的技术。[英文文摘]DNA polymorphism of arbuscular mycorrhizal fungi (AMF) was analyzed through the method of DNA amplification by nested PCR based on AFLP marker,in order to solve the difficulty of identifying the species of AMF associated with mei flowers (Prunus mume Sieb.et Zucc.).A total of 30 root samples from 18 mei cultivars were collected in the flowering phase from Wuhan Mei Garden as experimental materials.The results show that the purified DNA can be extracted only from eight root samples that account for 26.7% of total root samples.Totally 24 polymorphic loci were obtained from eight sample roots,averaging 3. 0 loci for each sample.The average genetic identity was 0. 409 7 ±0. 084 8,and the Shannon information index was 0. 596 8 ± 0. 095 5. The clustered groups of AMF DNA by AFLP marker from different cultivars were not identical with cultivar groups of P.mume.The results indicate that the AFLP marker technology is a brief and effective method to study the DNA polymorphism for the AMF in the roots of a plant.zh_CN
dc.description.sponsorship教育部科学技术研究重点项目(104034);; 国家自然科学基金项目(30670047);; 厦门市科技计划项目(3502Z20072010、3502Z20112004)zh_CN
dc.language.isozhzh_CN
dc.publisher《北京林业大学学报》编辑部zh_CN
dc.subject丛枝菌根真菌zh_CN
dc.subject巢式PCRzh_CN
dc.subject扩增片段长度多态性zh_CN
dc.subjectzh_CN
dc.subjectarbuscular mycorrhizal fungizh_CN
dc.subjectnested PCRzh_CN
dc.subjectamplified restriction fragment polymorphism (AFLP)zh_CN
dc.subjectPrunus mumezh_CN
dc.title梅根系丛枝菌根真菌AFLP分析zh_CN
dc.title.alternativeAFLP analysis of arbuscular mycorrhizal fungi in roots of Prunus mumezh_CN
dc.typeArticlezh_CN


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