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dc.contributor.authorZhu, Xuan
dc.contributor.author朱铉
dc.contributor.authorSu, Meiqin
dc.contributor.authorTang, Shaoheng
dc.contributor.authorWang, Lingsong
dc.contributor.authorLiang, Xinfang
dc.contributor.authorMeng, Feihong
dc.contributor.authorHong, Ying
dc.contributor.authorXu, Zhiran
dc.date.accessioned2013-03-08T07:28:17Z
dc.date.available2013-03-08T07:28:17Z
dc.date.issued2012-06-18
dc.identifier.citationMOLECULAR VISION,2012,18(200-10):1973-1982zh_CN
dc.identifier.issn1090-0535
dc.identifier.uriWOS:000306713200008
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/15082
dc.description.abstractPurpose: The goal of the present study was to synthesize mucoadhesive polymer - thiolated chitosan (TCS) from chitosan (CS), then prepared CS/TCS-sodium alginate nanoparticles (CS/TCS-SA NPs), determined which was more potential for ocular drug delivery. Methods: A new method for preparing TCS was developed, and the characteristics were determined using Fourier transform infrared spectroscopy and the degree of thiol immobilized was measured by Ellman's reagent. Human corneal epithelium (HCE) cells were incubated with different concentrations of TCS for 48 h to determine the cell viabilities. CS/TCS-SA NPs were prepared and optimized by a modified ionic gelation method. The particle sizes, zeta potentials, Scanning electron microscopy images, mucoadhesion, in vitro cell uptake and in vivo studies of the two types of NP were compared. Results: The new method enabled a high degree of thiol substitution of TCS, up to 1,411.01 +/- 4.02 mu mol/g. In vitro cytocompatibility results suggest that TCS is nontoxic. Compared to CS-SA NPs, TCS-SA NPs were more stable, with higher mucoadhesive properties and could deliver greater amounts of drugs into HCE cells in vitro and cornea in vivo. Conclusions: TCS-SA NPs have better delivery capability, suggesting they have good potential for ocular drug delivery applications.zh_CN
dc.description.sponsorshipScience and Technology Planning Projects of Xiamen Science & Technology Bureau, China [502Z20093012, 3502Z20103009]zh_CN
dc.language.isoenzh_CN
dc.publisherMOLECULAR VISIONzh_CN
dc.subjectIN-VITRO EVALUATIONzh_CN
dc.subjectTHIOMERSzh_CN
dc.subjectPOLYMERSzh_CN
dc.subjectCARRIERSzh_CN
dc.subjectSYSTEMzh_CN
dc.titleSynthesis of thiolated chitosan and preparation nanoparticles with sodium alginate for ocular drug deliveryzh_CN
dc.typeArticlezh_CN


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