• 中文
    • English
  • English 
    • 中文
    • English
  • Login
View Item 
  •   DSpace Home
  • 生命科学学院
  • 生命科学-已发表论文
  • View Item
  •   DSpace Home
  • 生命科学学院
  • 生命科学-已发表论文
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Gambogic acid activates AMP-activated protein kinase in mammalian cells

Thumbnail
Full Text
Gambogic acid activates AMP-activated protein kinase in mammalian cells.htm (413bytes)
Date
2012-07-20
Author
Zhao, Baobing
Shen, Huili
Zhang, Lianru
Shen, Yuemao
沈月毛
Collections
  • 生命科学-已发表论文 [5878]
Show full item record
Abstract
AMP-activated protein kinase (AMPK) plays a key role in maintaining intracellular and whole-body energy homeostasis. Activation of AMPK has been shown to ameliorate the symptoms of metabolic diseases, such as type 2 diabetes and obesity. Here we show that gambogic acid (GB), a known antitumor agent, activates AMPK by increasing the phosphorylation of AMPK alpha and its downstream substrate ACC in various cell lines. Further study revealed that GB stimulated AMPK activity independent of upstream kinases. Moreover, the AMPK inhibitor, compound C, has no effects on the GB-induced AMPK activation. We also found that GB promptly increased intracellular ROS level, and antioxidants attenuated the ROS production. Interestingly, only the thiol antioxidants significantly abolished GB-enhanced AMPK activation. In addition, analysis of binding and dissociation kinetics indicated that GB bound to the AMPK alpha. subunit. Collectively, these results suggest that GB may be a novel direct activator of AMPK. (C) 2012 Elsevier Inc. All rights reserved.
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2012,424(1):100-104
URI
http://dx.doi.org/10.1016/j.bbrc.2012.06.078
WOS:000307135700018
https://dspace.xmu.edu.cn/handle/2288/15079

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies
 

 

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies