ANALYSIS ON GENETIC DIVERSITY OF SEVEN MANGROVES IN RHIZOPHORACEAE IN CHINA
- 生命科学－已发表论文 
[中文文摘]以红树、红海榄、秋茄、角果木、木榄、海莲、尖瓣海莲等 7种红树科植物为材料 ,采用改进的CTAB法获得了纯度较高、得率高、片段完整的基因组DNA。通过筛选出的 1 5个有效引物进行RAPD分析 ,探讨了 7种红树植物间的亲缘关系。1 5个有效引物共扩增出 61 7条DNA带 ,其中多态性条带 41 5条 ,占总扩增条带的 67 2 6%。利用Nei指数法得出 7个分类群间的遗传一致度和遗传距离 ,并运用UPGMA法进行聚类分析。 7个分类群分为A、B两个大组 ,平均遗传距离为 0 41。将得出的 7个分类群的DNA分子分类系统图 ,与传统的分类进行比较 ,发现结果相符。同时获得一个OPG0 5— 90 0的差异片段可作为区分海莲和尖瓣海莲的分子标记。[英文文摘]DNAs from 7 txax of mangroves in Rhizophoraceae were extracted by using the CTAB method. A 260 /A 280 ratio of DNA solutions ranged from 1.6 to 2.0,and the length of DNA fragment was about 23kb. RAPD markers were used to assess the relationships among the 7 taxa of mangroves in Rhizophoraceae. Fifteen effective primers were screened from 30 10-oligonucleotide arbitrary primers,and a total of 617 DNA bands were amplified,among which 415 (67.26%) were polymorphic. Based on UPGMA cluster analysis of 617 DNA bands amplified by the 15 primers, a DNA molecular dendrog ram was established, which d-ivided 7 species in Rhizophoraceae into 2 main groups. The average genetic distance among the 7 taxa was 0. 41, and the maximum genetic distance was 0. 56( < 0. 6) , which between Ceriops tagal and Bruguiera sex angula var. rhy nchopetala. It indicated that the 7 taxa are in the same family. Group A included 3 taxa- R hiz ophora apiculata, R . stylosa , Ceriops tagal; group B contained 4 species Bruguiera gymnorr hiza, B . sex angula, B. sexangulavar . rhynchop etala, and K andelia candel . The dendrogram obtained from the cluster analysis was consistent to morphological classification. A special DNA f ragment , which named OPG05-900, was amplified to distinguish B. sex angula and B . sex angulavar . rhynchopetala. The results could be valuable to the projects of plant introduction, ecological restoration and conservation in mangroves.