Genetic Analysis and Mapping of Rice(Oryza sativa.L) sd-sl Mutant
- 生命科学－已发表论文 
[中文文摘]新的矮秆基因的发掘、研究和利用对水稻育种和植物生长发育机制研究有重要的作用.用60Coγ射线辐照粳稻9522,获得一个能稳定遗传的突变体.该突变体表型为株高较野生型矮,叶片短而微卷.将该突变体与籼稻广陆矮杂交,F2代呈3∶1分离,说明该突变体受隐性单基因控制.通过InDel分子标记对F2代分离群体进行遗传定位,将该基因定位于第6染色体InDel标记OS604附近.随后又发展了多对有多态性的InDel分子标记,将该基因座位精细定位在InDel标记XL6-6和XL6-1之间,AP003490和AP005619上,两个引物之间的物理距离为118 kb.本研究为该克隆基因及其作用机理的探究奠定了基础.[英文文摘]It is the bright road of rice breeding to study about dwarf mutant . On the other hand ,dwarf mutant s in rice are crucial for elucidating regulating mechanisms for plant growth and development . In the present study ,the j aponica cultivar 9522 was muta-genized by 60Coγ-Ray and then a rice dwarf mutant s d2sl was found which was dwarf and had smallish ,olled leaves. Genetic analysis indicated that the phenotype was cont rolled by a single recessive gene. To map sd-sl locus ,a F2 population was const ructed f rom the cross between the s d2sl ( j a ponica) and guangluai ( indica) . Firstly ,the s d2sl locus was roughly mapped by InDel marker ,OS604 ,in rice chromosome 6. And then several polymorphic markers were developed for further fine2mapping. At last the sd-sl locus was mapped between the two InDel markers ,XL626 and XL621. The region was delimited to about 118 kb. These result s provide a basis for molecular cloning and functional analysis of sd-sl .