大黄鱼血清抗病功能蛋白分析

Abstract

[中文文摘]利用蛋白质组学技术筛选大黄鱼(Pseudosciaena crocea)血清中的抗病功能蛋白,通过对比两组抗病能力差异大的大黄鱼血清的双向电泳图谱,得到了21个显著的差异点,并用质谱获得上述差异点肽质量指纹图谱.进一步检索发现10号差异点是诱导型一氧化氮合成酶(iNOS),其催化产物NO可结合病原体的特定靶点,协助大黄鱼体内的免疫系统共同抵御病原体入侵,显著提高了大黄鱼的抗病能力,所以iNOS与大黄鱼抵抗病原体入侵密切相关.[英文文摘]The control group and experimental group,between which there were marked difference in the protection rate,were analyzed by using proteomic technology in order to find out antidisease protien in serum sample of Pseudosciaena crocea.21 different ｓpots in serum sample were found through comparing the 2-DE gel maps of control group with experimental group.These different spots were analzed by PMF (peptide mass fingerprint) through MALDI-TOF-MS.One of the spots was iNOS(inducible nitric oxide synthase,EC 1.14.13.39).As one of the iNOS's catalytic products,NO bonded with the specific target of pathogens.It assisted the immune system of Pseudosciaena crocea to resist the invasion of pathogens.No significantly improved the Pseudosciaena crocea′ｓdisease resistance.So INOS may be an importent role in antidisease mechanism of Pseudosciaena crocea.