Now showing items 1-10 of 114

    • N-(Z)-9-十八烯基-2-丙磺酰胺对2型糖尿病小鼠胰岛素抵抗的影响及其机制 

      任桐; 林辰曦; 刁勇 (2017)
      探索新化合物N-(Z)-9-十八烯基-2-丙磺酰胺(N15)对2型糖尿病(T2DM)胰岛素抵抗小鼠的影响并探讨其可能作用机制。采用链脲佐菌素(S; TZ)连续小剂量腹腔注射诱导T2DM小鼠模型, N15(50、100和200 mg·kg~(-1)·d~(-1))和吡格列酮(6; mg·kg~(-1)·d~(-1))连续灌胃给药6周,期间分别对小鼠空腹血糖(FBG)、胰岛素(FIns)和胰岛素抵抗指数(HOMA-IR)进行; ...
    • 稳定干扰AGR2乳腺癌细胞系的建立及其功能 

      邓鸣涛; 李飞飞; 吕忠显 (2011)
      将Agr2基因的SHrnA表达序列连接到PSuPEr质粒中,获得PSuPEr-SHAgr2质粒,经测序鉴定后,将PSuPEr-SHAgr2和PSuPEr质粒通过lIPOfECTAMInE2000转染MCf7细胞,经流式细胞仪和600μg.Ml-1g418筛选获得稳定干扰Agr2的MCf7细胞系和对照细胞系,WESTErn blOT检测其rnA干扰效果,MTT检测细胞生长情况;用无血清酚红培养基饥饿12 H后,25 ng.Ml-1Egf处理15 ...
    • SNX12调控BACE1的细胞内定位和Aβ的产生 

      王云姝; 赵永浩; 赵颖俊; 张弦; 许华曦; 张云武 (2011)
      不溶性β淀粉样蛋白(β-AMylOId,Aβ)的沉积是阿尔茨海默症(AlzHEIMEr′S dISEASE,Ad)的中心环节,Aβ是由β-分泌酶(bACE1)和γ-分泌酶顺序切割β淀粉样蛋白前体蛋白(β-AMylOId PrECurSOr PrOTEIn,APP)产生的,bACE1与APP在质膜、内含体/溶酶体、高尔基体反面膜囊(Tgn)以及早期分泌途径之间的转运很大程度上影响了Aβ的产生.通过WESTErn blOT和免疫荧光的方法探讨了SOrTIng ...
    • 脑特异性高表达蛋白TMEM59L诱导细胞凋亡的机制 

      钱凌芝; 赵永浩; 谢勇壮; 郑秋阳; 张弦; 许华曦; 张云武 (2011)
      TMEM59l为近年来新发现的脑特异性高表达蛋白,具有促凋亡效果,但其具体的凋亡机制还不清楚.构建了TMEM59l重组质粒,并在HEk293T细胞中过表达TMEM59l,用AnnEXIn V-fITC/PI双染法确定了TMEM59l可以诱导细胞凋亡,之后用免疫印迹方法检测细胞内凋亡相关分子激活情况.结果显示,外源TMEM59l可以降低bCl-2的蛋白表达水平,诱导细胞色素C从线粒体释放进入细胞质,并且激活CASPASE-9、CASPAS ...
    • FAM105A通过caspase依赖性途径和Bcl-2家族诱导细胞凋亡 

      袁梦; 赵永浩; 王云姝; 刘亦倩; 张弦; 许华曦; 张云武 (2011)
      在多细胞有机体中,细胞凋亡对调节正常细胞活动和发育起着关键作用.对参与细胞凋亡的蛋白的鉴定及其机制的阐明具有重要意义.fAM 105 A是新发现的促凋亡基因,过表达会导致细胞凋亡.研究了fAM105A促进细胞凋亡的潜在机制,结果表明,在HEk293T细胞中过表达fAM105A会导致CASPASE-3和CASPASE-9的剪切/激活,同时伴随着多聚AdP-核糖聚合酶(POly AdP rIbOSE POlyMErASE,PArP)的切割/ ...
    • 25-羟基维生素D_3人工完全抗原的合成与鉴定 

      万巍巍; 张换敬; 叶辉铭; 万丽琴; 蔡良良; 郑立谋; 曾骥孟 (2011)
      目的:合成25-羟基维生素d3人工完全抗原,并制备抗25-羟基维生素d3的特异性抗体。方法:将25-羟基维生素d3进行化学修饰加入羧基活性基团,合成具有半抗原结构特征的25-羟基维生素d3-半琥珀酸酯。采用碳二亚胺法,将25-羟基维生素d3-半琥珀酸酯,分别与牛血清白蛋白(bSA)和卵清蛋白(OVA)偶联,合成人工完全抗原25-羟基维生素d3-半琥珀酸酯-bSA和25-羟基维生素d3-半琥珀酸酯-OVA。通过紫外吸收光谱,SdS-PAg ...
    • Quantification and visualization of glutathione S-transferase omega 1 in cells using inductively coupled plasma mass spectrometry (ICP-MS) and fluorescence microscopy 

      Liang, Yong; Jiang, Xin; Tang, Nannan; Yang, Limin; Chen, Haifeng; Wang, Qiuquan; 陈海峰; 王秋泉; 杨利民 (Springer Verlag, 2014 Novem)
      We report a novel activity-based and Cu-free click chemistry (CC) mediated methodology for glutathione S-transferase omega 1 (GSTO1) quantification using species-unspecific isotope dilution inductively coupled plasma mass ...
    • Formation of monodisperse mesoporous silica microparticles via spray-drying 

      Waldron, Kathryn; Wu, Winston Duo; Wu, Zhangxiong; Liu, Wenjie; Selomulya, Cordelia; Zhao, Dongyu; Chen, Xiao Do; 刘文杰; 陈晓东 (ACADEMIC PRESS INC ELSEVIER SCIENCE, 2014 March)
      In this work, a protocol to synthesize monodisperse mesoporous silica microparticles via a unique microfluidic jet spray-drying route is reported for the first time. The microparticles demonstrated highly ordered hexagonal ...
    • Myrotheciumones: Bicyclic cytotoxic lactones isolated from an endophytic fungus of Ajuga decumbens 

      Lin, Ting; Wang, Guanghui; Shan, Wenjun; Zeng, Dequan; Ding, Rong; Jiang, Xin; Zhu, Dan; Liu, Xiaoxuan; Yang, Shuiyuan; Chen, Haifeng; 林挺; 杨水源; 陈海峰 (PERGAMON-ELSEVIER SCIENCE LTD, 2014-06-01)
      Two new bicyclic lactones, myrotheciumones A (1) and B (2) which possessed a rare ring-fusion system were isolated from Myrothecium roridum (M. roridum), an endophytic fungus of the medicinal herb plant Ajuga decumbens (A. ...
    • Total synthesis and RXR alpha-mediated transcription studies of neriifolone B and related compounds 

      Shen, Qirong; Dai, Yi; Wang, Guanghui; Yao, Fei; Duan, Yinghui; Chen, Haifeng; Zhang, Weige; Zhang, Xiaokun; Yao, Xinsheng; 王光辉 (PERGAMON-ELSEVIER SCIENCE LTD, 2014-05-01)
      Neriifolone B (1), a natural product containing a novel 4',4'-dimethyl-4',5'-dihydropyran-6-one[2',3':3,4]xanthone skeleton, was found to be a potent inhibitor of transcription mediated by retinoid X receptor alpha (RXR ...