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dc.contributor.authorBai, Rongyao
dc.contributor.authorYou, Weiwei
dc.contributor.authorChen, Jun
dc.contributor.authorHuang, Heqing
dc.contributor.author黄河清
dc.contributor.authorKe, Caihuan
dc.contributor.author柯才焕
dc.date.accessioned2013-01-08T09:03:26Z
dc.date.available2013-01-08T09:03:26Z
dc.date.issued2012-10
dc.identifier.citationFISH & SHELLFISH IMMUNOLOGY,2012,33(4):81-96zh_CN
dc.identifier.issn1050-4648
dc.identifier.urihttp://dx.doi.org/10.1016/j.fsi.2012.05.003
dc.identifier.uriWOS:000310185800001
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/14467
dc.description.abstractGABA(A) receptor-associated protein (GABARAP), a multifunctional protein participating in autophagy process, is evolutionarily conserved and involves in innate immunity in eukaryotic cells, but currently there is no research on the relationship between GABARAP and innate immunity in mollusc. In the present study, the GABARAP full-length cDNA and its genomic DNA were firstly cloned from small abalone (Haliotis diversicolor), which was named as saGABARAP Its full-length cDNA is 963 bp with a 354 bp open reading frame encoding a protein of 117 aa, a 276 bp 5'-UTR, and a 333 bp 3'-UTR including a poly(A) tail, two typical polyadenylation signals (AATAA) and two RNA instability motifs (ATTTA). The deduced protein has an estimated molecular weight of 13.9 kDa and a predicted PI of 8.73. Its genomic DNA comprises 4352 bp, containing three exons and two introns. Quantitative real-time PCR analysis revealed that saGABARAP was constitutively expressed in all examined tissues, with the highest expression level in hepatopancreas, and was upregulated in hepatopancreas and hemocytes after bacterial challenge. In addition, saGABARAP was ubiquitously expressed at all examined embryonic and larval development stages. These results suggested that saGABARAP could respond to bacteria challenge and may play a vital role in the adult innate immune system against pathogens and the development process of abalone embryo and larvae. (C) 2012 Elsevier Ltd. All rights reserved.zh_CN
dc.description.sponsorshipHi-Tech Research and Development (863) Program of China [2012AA100812]; National Natural Science Foundation of China [31101896, 40976093]; earnmarked fund for Modern Agro-industry Technology Research System [nycytx-47]; program for Changjiang Scholars and Innovative Research Team in the Xiamen University [IRT0941]zh_CN
dc.language.isoenzh_CN
dc.publisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTDzh_CN
dc.subjectGABARAPzh_CN
dc.subjectHaliotis diversicolorzh_CN
dc.subjectAutophagyzh_CN
dc.subjectInnate immunityzh_CN
dc.titleMolecular cloning and expression analysis of GABA(A) receptor-associated protein (GABARAP) from small abalone, Haliotis diversicolorzh_CN
dc.typeArticlezh_CN


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