Study on Effect of Total Sn Contents and Bioconcentration inHaliotis diversicolor supertexta Blood Exposed to Tribulyltin Chloride
- 环境生态－已发表论文 
用氯化三丁基锡分别在0、0.01、0.1、1、10和100ng/L浓度下对杂色鲍(Haliotisdiversicolorsupertexta)进行暴污和恢复,在不同时间(第2、6、14、26、44、64d以及恢复期第8、20d)取血液样.用微波消解样品、ICP MS法测定其血液中总锡的含量,并计算总锡的生物富集系数,结果表明,随着三丁基锡浓度的上升,血液中总锡的含量也升高,100ng/L组在44d总锡含量就达到了最高;在20d恢复期内0.01ng/L浓度组总锡浓度继续保持缓慢上升,0.1ng/L浓度组总锡浓度基本不变,其余浓度组总锡浓度有所下降但无法恢复到对照组的水平,各浓度组总锡的生物富集系数随着浓度的上升反而降低.本文揭示了杂色鲍的血液是锡的蓄积库之一.The effects of tribulyltin chloride (TBT) on total Sn contents and bioconcentration inHaliotis diversicolor supertexta blood were studied with single doses of 0.01,0.1,1,10,100 ng /L under the experimental ecological conditions. Blood samples were taken after exposure for 2 d, 6 d, 14 d, 26 d, 44 d, 64 d and recover 8 d, 20 d respectively, digested with microwave and measured with ICP-MS, then Bioconcentration Factors(BCF) were calculated. The results showed that the total Sn contents in blood was elevated with the increasing concentration of TBT, 100 ng/L group reach the top on 44th day, and other groups did on 64th day. In the 20 d recover period, the total Sn contents increased slowly in 0.01 ng/L group, and held the line in 0.1 ng/L group, and reduced in other groups, but didn't reach the control level. The BCF of total Sn contents in blood was reduced in the increasing concentration group. The BCF were as large as 10~4～10~7.H.diversicolor blood was one of storage pool for Sn. That indicated TBT and its metabolite were accumulated strongly byH.diversicolor, and the toxic effects of TBT were pricked up consequently. The method determined by microwave digestion-ICP-MS is simple and accurate.