Rapid Quality Control of Licorice in Its GAP Research
- 化学化工－已发表论文 
目的 建立甘草 GAP过程中的快速质量检验方法 ,为甘草药材的快速质量检测提供方法依据。方法 提出甘草中水溶性有效物质群的概念并建立其含量的比色测定方法。以甘草酸作为定量的标准物质 ,采用香草醛 -浓硫酸试剂作显色剂 ,用便携式小型光谱仪于 5 6 0 nm处检测。结果 回归方程为 Y=1.174 7C+0 .0 873,相关系数 r=0 .970 5 (n=3) ,回收率为 92 %～ 99% ,在 0 .0～ 0 .96 mg/m L 范围内仪器读数与甘草酸浓度呈线性关系。结论 该方法简单、快速、廉价 ,能够准确地反映甘草质量的优劣 ,适合在准确度要求相对较低的甘草生产基地推广使用Objective To develop a rapid method for the quality control of licorice in its GAP research.Methods Water-soluble triterpenoid saponins were defined as the active fraction in licorice and a colorimetric method using LED-based portable device has been developed for the first time to measure the content of this active fraction. The method was based on the specific color reaction occurring between triterpenoid saponins and vanillin-sulfuric acid reagent. Visible light absorption of 560 nm was chosen as the detection wavelength.Results The operating calibration curve(Y=1.174 7C +0.087 3 mg/mL) was found to be linear at the range of 0.0～0.96 mg/mL glycyrrhizic acid in water (r=0.970 5). The recovery is 92～99%.Conclusion The method is simple, rapid and low-cost and is recommendable to evaluate the quality of Glycyrrhiza uralensis Fisch. in its GAP research.