Determination of Stevia Main Component Stevioside by Capillary Zone Electrophoresis
- 化学化工－已发表论文 
采用 Tris-硼砂作为毛细管电泳分离测定甜菊苷的新体系 ,探讨了进样量、温度、电压、缓冲液浓度以及 p H值等条件对甜菊苷分离效果、灵敏度及分离时间的影响 .通过优化各种实验条件 ,建立一种毛细管区带电泳分离甜菊苷的新方法 .实验结果表明该方法具有如下优点 :1)快速 :甜菊中两种主要甜味成分在 3.5min内均达到完全分离 ;2 )灵敏度高 :最小检测浓度可达 6.2× 10 -5mol/ L;3)分离效率高 :理论塔板数高达 3.3× 10 4 / m;4 )稳定性好 :St出峰时间的 R.S.D为 0 .71% ,RA为0 .4 6% .Stevioside在 0 .5～ 7.0 mg/ m L的浓度范围内存在良好的线性关系 .实验实现了甜菊叶提取物中甜菊苷的分离测定 ,结果令人满意 .This paper introduced Tris sodium tetraborate as novel electrophoretic buffer for stevia separation. The effect of different separation conditions on the migration time, selectivity and resolution was investigated, including the injected amount of sample, voltage, capillary temperature, concentration and pH value of buffer system. The characters of this method were listed below: 1)Rapid, stevioside and rebaudioside A were provided good resolution in 3.5 min; 2)High sensitivity; 3)Efficient, the theoretical plate of St was 3.3×10 4 /m; 4)The R.S.D for the migration time of Stevioside and Rebaudioside A were 0.71% and 0.46%, respectively. The detector response for Stevioside was linear over the range 0.5~7.0 mg/mL. Stevioside in stevia extract was satisfactorily quantified by this method.