Determination of Microamouts of Ammonia in Meat by Nessler's Reagent Spectrophotometry
- 化学化工－已发表论文 
本文提出了一种测定肉中粗氨的方法,可用于肉品新鲜度的检验。在常温下,纳氏试剂与肉中粗氨反应生成淡红棕色胶态化合物,最大吸收波长为400nm,表观摩尔吸光系数为1.404×104L/mol·cm,线性方程为y=0.52x+0.0001,r=0.9997,线性范围为0～1.0μg/ml,回收率在97.8%～104.8%之间。A method for the determination of the crude ammonia in meat was presented in this paper. The method based on the color reaction of ammonia-Nessler's Reagent in room temperature. The results showed that the apparent molar absorptivity is 1.404 ×104 L/mol·cm at the wavelength of 400nm. Beer's law is obeyed for the ammonia in the range of 0～1.0 μg/ml. The correlation coefficient is 0.9995. The recovery rate is 97.8%～104.8%. The method has been applied satisfactorily to crude ammonia in meat.