Thermodynamics of Basic Compounds on Novel Stationary Phase Embed Polar Groups
- 化学化工－已发表论文 
以苯、吡啶、尼古丁和普鲁卡因为探测因子,考察了酰胺型、胺型、醚型和酯型4种“包埋”极性官能团的新型键合固定相对碱性化合物在流动相pH值分别为3·0和7·0时的热力学焓变。实验结果表明,新型固定相由于烷基分子链中“嵌入”极性官能团,因此在流动相pH为3·0时温度对碱性溶质的保留影响相互差异较大。在流动相pH为7·0时,根据Van′t Hoff方程计算出4种待测物质从流动相转移到固定相上的ΔH0,从ΔH0的绝对值大小、相关系数以及实际的分离效果可以发现,在分离强碱性物质时,若实验条件一样,则分离效果是酰胺型和胺型>酯型固定相>醚型固定相,但它们均优于传统的固定相。The thermodynamis character of three basic probes and a neutral reference compound were investigated as a function of temperature using four novel high performance liquid chromatography columns that are amide-type、 amine-type and ester-type stationary phases at both pH 3.0 and pH 7.0. The results showed that considerable differences of retention for bases at pH 3.0 might result from the existence of polar group in the long chain alkyl chain on the packing. The ΔH~0 values of four probes on four novel columns and a conventional C_8 column were calculated according the van Deemter curves. The separation results of basic samples on the novel columns and C_8 column shold amide-type and amine-type exceed ester-type and ether-type, but their all exceed conventional C_8 column according to absolute values of ΔH~0 , correlation coefficient and practical separation.