Analysis of Nitrate Reducing Community in a Near-shore Marine-cultural Sediments
- 海洋环境－已发表论文 
通过对福建省沿海海水养殖场沉积物中参与氮循环的各生理群细菌数量分析 ,发现氨化和硝酸盐还原细菌是优势生理菌群 ,同时 ,表层泥样中的硝酸盐还原菌数量明显高于深层泥样。从该环境中分离获得 1 0 6株细菌 ,其中 5 8株具有硝酸盐还原能力 ,初步鉴定表明它们主要为芽孢杆菌属 (Bacillus)、盐芽孢杆菌属 (Halobacillus)、短芽孢杆菌属 (Brevibacil lus)、动性球菌属 (Planococcus)和动性杆菌属 (Planomicrobium)等革兰氏阳性细菌的成员 ;1 6SrRNA基因序列分析进一步证实该环境中的硝酸盐还原菌具有丰富的多样性The nitrogen-cycling bacteria in a near-shore marine-cultural sediments were investigated. Results indicated that ammonifying and nitrate-reducing bacteria (NRB) were the most abounding populations. The abundance of nitrate-reducing bacteria occurred in the surface layer of the sediment was higher than that in the bottom layer. 106 bacterial strains were obtained from sediment samples and were tested for their nitrate reducing ability. The results showed that 58 strains of them were able to reduce nitrate. The dominant nitrate-reducing strains were preliminarily identified as Gram positive bacteria and belong to the genus of Bacillus, Halobacillus, Brevibacillus, Planococcus and Planomicrobium.The richness of diversity of nitrate-reducing bacteria was further revealed by the analysis of the sequences of their 16S rRNA genes.