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dc.contributor.author陈仕玺
dc.contributor.author洪万树
dc.contributor.author张其永
dc.contributor.author马细兰
dc.contributor.author倪子绵
dc.date.accessioned2017-11-14T01:24:44Z
dc.date.available2017-11-14T01:24:44Z
dc.date.issued2004-10-30
dc.identifier.citation中国水产科学,2004,(05):12-19
dc.identifier.issn1005-8737
dc.identifier.otherZSCK200405003
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/141602
dc.description.abstract应用组织学和透射电镜研究雄性大弹涂鱼(Boleophthalmuspectinirostris,Linnaeus)贮精囊的形态结构。结果表明:(1)贮精囊是其精巢的1对附属腺体,黑色素分布于翼状贮精囊表面;(2)贮精囊外包结缔组织被膜,并向内伸出结缔组织隔膜,被膜和隔膜中含有间质细胞,平滑肌纤维、毛细血管、成纤维细胞和纤维细胞;(3)结缔组织隔膜将贮精囊分隔成为许多小室腔,被膜和隔膜上排列着单层上皮细胞,不同小室腔的上皮细胞发育并不同步,按外形分为柱形和立方形。小室腔内还有片状分泌物,PAS呈阳性反应,提示分泌物为粘多糖蛋白;(4)电镜显示上皮细胞顶部有微绒毛结构。生殖期间上皮细胞内粗面内质网、管状嵴线粒体和高尔基复合体发达,上皮细胞顶部有无膜包裹的分泌颗粒。排精后细胞器退化,胞质中出现大量的大空泡;(5)在生殖高峰期不论贮精囊的近端、中央或远端均先后贮存大量的精子,并混合在分泌物中。
dc.description.abstractThe mudskipper (Boleophthalmus pectinirostris), which inhabits intertidal zones, is an economically important species of marine fish cultured along the coastal areas in southeastern China. However, the supply of fry was completely dependent on capture from intertidal zones, and the number of wild fry has been gradually declining. Therefore, the establishment of a method of artificial propagation for the mudskipper was needed to stabilize the supply. Maturation of the 4th phase oocytes of mudskipper was induced in vitro by pheromones in the extract of the seminal vesicles. The present study attempted to describe the morphological and structural characteristics of the seminal vesicles in male mudskipper by means of histology and transmission electron microscope. Live specimens of male mudskipper,8.2-11.9 cm in body lengths and 9.0-29.6 g in body weigths, were collected from the intertidal zones of Jiulong River and Quanzhou Bay, Fujian Province. The animals were dissected to remove the testes and seminal vesicles together with the sperm duct. The lengths and weigths of these organs were measured and taken photos by a SONY-digital camera. Some of the seminal vesicles were fixed in Bouins solution for light microscopy. For electron microscopy, other seminal vesicles were fixed in 2.5% phosphate buffered glutaraldehyde, followed by post-fixation in osmium tetroxide and embedding in Epon. Light microscopic sections were observed under an Olympus DX51 microscope and photographed by an Olympus DP11 digital camera, whereas the transmission electron micrographs were obtained with a JEM-100CXⅡ. The results showed that the seminal vesicles were a paired structure which is situated posterior to the testis along the whole length of the nontesticular part of the sperm ducts. Both sperm ducts join to form an unpaired portion which opened on the genital papilla with bladder. Each vesicle was wing-like in shape, opaque and with melanins on exterior. The maximal widths of the left part and the right part were 2-3 mm and 2.5-4 mm, respectively. The lengths of the left part and the right part were 9-11 mm and 8.5-11.5 mm, respectively. Histological sections of the seminal vesicles revealed that the organ was enclosed by connective tissue capsula, and connective tissue partitions were prolonged inside. Both capsula and partition were consisted of smooth muscle fibers, blood capillaries, fibroblasts, fibrocytes and interstitial cells. The thickness of the connective tissue partitions and connective tissue capsula was 3.5-20.9 μm and 6.9-10.4 μm, respectively. The seminal vesicle was divided into many locule lumina, which were 66.1-426 μm in diameter, by the connective tissue partitions. Single epithelial cells, which were columnar and cuboidal in shape, arranged along the partition. Bubble-like secretion, which connected to slice-secretion, were at top of the epithelial cells. The columnar cells were 22.6-37.2 μm in heights and 4.5-5.3 μm in widths. The cuboidal cells were 13.3-16.9 μm in heights and 3.9-4.3 μm in widths. Slice-like secretion of the seminal vesicles showed PAS positive reaction in dark purple-red colour, suggesting that the secretion should be mucopolysaccharide-mucin. Electron microscopic observation showed that there were microvilli at top of the epithelial cells. The microvilli were 1.37-1.85 μm in lengths and 33-41 nm in diameters. Many secretory granules without membranes accumulated to the apical portion of the epithelial cell. During the spawning season, rough endoplasmic reticulum, tubular cristae mitochondria and Golgi complex were well developed in the epithelial cells. The cell organelles degenerated and large vacuoles appeared after the seretory granules were released. During the breeding season the seminal vesicles were turgid, more or less conical in shape and the connective tissue partitions and connective tissue capsula were thinner than those in the post-breeding season. At the peak period of spawning, a large number of spermatozoa, which were mixed with the secretion, appeared in proximal, middle and
dc.description.sponsorship国家自然科学基金资助项目(30170739;40276040);; 福建省自然科学基金资助项目(B0210003).
dc.language.isozh_CN
dc.subject大弹涂鱼
dc.subject贮精囊
dc.subject形态结构
dc.subject超微结构
dc.subjectBoleophthalmus pectinirostris
dc.subjectseminal vesicles
dc.subjectmorphological structure
dc.subjectultrastructure
dc.title雄性大弹涂鱼贮精囊的形态结构
dc.title.alternativeMorphology and structure of the seminal vesicle in male Boleophthalmus pectinirostris
dc.typeArticle


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