Isolation and identification of PAH-degradating strains and their degradation capability
- 海洋环境－已发表论文 
以芴、菲、蒽、芘为碳源和能源筛选、分离PAHs降解菌。14株能降解利用PAHs的菌株被分离。通过HPLC分析,在含芴、菲、蒽、芘的混合培养基质中10号菌的降解能力最强。研究它的降解性能和生长情况,表明该菌在混合反应体系中培养30d后对芴、菲、蒽、芘的降解率分别为95.27、90.46、28和80%;在只含一种PAH的单反应体系中该菌对芴、菲、蒽的降解能力提高,降解率分别可达98.91、94.32和52.17%,而对芘的降解能力则降低,降解率仅为62.47%。与混合PAHs培养体系相比,在单一PAH培养体系中,细菌的对数生长期缩短1/3。经生理生化鉴定和16SrDNA序列对比分析,确定10号菌株属于假单胞菌,命名为PseudomonasspFAP10。Fluorenen, phenathalene, anthracene and pyrene were used as carbon and energey source to isolate and screen PAHs-degrading strains. Fourteen strains capable of degrading PAHs were isolated from the petroleum in seawater contaminated. Analyses was done by HPLC, strain No.10 showed the strongest degrading ability in the mixing system of fluorene、phenathalene、anthracene and pyrene. Its degradation performance and growth curve were tested. The degradation rates of fluorenen, phenathalene, anthracene and pyrene were 95.27%、90.46%、28% and 80% respectively in the mixing system within 30d. Under the condition of single PAH existence,the degradation rates of fluorene、phenathalene and anthracene by strain No.10 increased to 98.91%、94.32%、52.17% respectively while the degradation rate of pyrene decreased to 62.47%. Compared to the mixing system, the logarithmic growth phase of strain No.10 was shortened one third in single PAH system.. Based on the results of physio-biochemical identification and phylogenetical analyses of 16S rDNA sequence, strain No.10 was belonged to Pseudomonas sp,named as Pseudomonas sp FAP10.