Determination of dissolved tryptophan in seawater with spectrofluorimetry
- 海洋环境－已发表论文 
本文初步建立了直接测定海水中溶解色氨酸的方法,并进行各种条件影响实验.在0～0.070mg/dm3浓度范围内色氨酸荧光强度与浓度之间线性关系良好.在最佳实验条件下,方法检测限为7.6×10-4mg/dm3,精密度小于3.1%,回收率86%～120%.A method for direct determination of soluble tryptophan in seawater was primarily established in this paper, and the experiments various on operationconditions of this method were investigated. The calibration curve for the relation between the fluorescent intensity and concentration of tryptophan showed a straight line in the concentration range of 0～0.070mg/dm~3. Under the optimizedconditions, the limit of detection was 7.6×10~(-4)mg/dm~3, the relative standard deviations were lower than 3.1% (n=6), and the recovery retes were 86%～120%. This method can be used directly for the determination of tryptophan in seawater.