A Novel hepcidin-like Antimicrobial Peptide Isolated from the Japanese Bass(Lateolabrax japonicus Cuvier et Valenciennes)
- 海洋环境－已发表论文 
利用分子生物学技术,从我国海水养殖鲈鱼(Lateolabraxjaponicus)分离到一种hepcidin抗菌肽新基因,并在国际GenBank公布(accessionnumber为AY547281).初步研究结果表明,在我国鲈鱼分离到的hepcidin基因属于hep cidin抗菌肽基因家族的新成员.A novel hepcidin-like antimicrobial peptide gene was isolated from the liver RNA of the Japanese bass(Lateolabrax japonicus) following bacterial challenge. Sequencing analysis of the novel hepcidin-like antimicrobial peptide showed that it shared eight cysteines at conserved positions identical to the human hepcidins and the hybrid striped bass(Morone chrysops×M.saxatilis) hepcidin. Peptide sequence and the predicted gene organization of the preprohepcidin were similar to that reported from the hybrid striped bass and human. A predicted 88-residue hepcidin prepropeptide consists of three domains: a signal peptide(24 amino acids), a prodomain (43 amino acids),and a mature peptide(21 amino acids). In contrast to the human and bass hepcidins,there were around 8 and 5 amino acids different in the predicted mature peptide,respectively.The pioneer research indicated that the hepcidin from the Japanese bass was the homologue of the hybrid striped bass and human hepcidins, meaning that a new member of the hepcidin family was discovered.