Response of pyrene and 1-pyrenol concentrations in weever's bile to pyrene exposure in seawater
- 海洋环境－已发表论文 
运用GC MS的方法和荧光分光光度法,研究鲈鱼分别暴露于0.1、1.0和10.0μg/dm3质量浓度芘溶液中7d,水体中芘及其鲈鱼胆汁中芘和1 羟基芘含量的变化.实验结果显示:(1)鲈鱼对水体中的芘具有非常显著的去除作用.(2)随着芘暴露浓度的增大,鲈鱼对芘的代谢去除作用增强.(3)随着暴露时间的延长和芘暴露浓度的增大,胆汁的1 羟基芘浓度递增.(4)鲈鱼胆汁具有较高的芘浓度,对海水中的芘具有较强的富集作用.(5)鲈鱼胆汁的1 羟基芘及芘的浓度与水体芘浓度均具有很好的相关性,对于指示水体的芘污染程度具有一致性;可作为指示水体芘污染程度的生物标志物.GC/MS and fluorescence methods were used to study the responseof weever's bile to pyrene exposure in a short period of time (7 days) at concentrations of 0.1～10.0μg/dm~3 in experimental condition. The results showed that: (1)weever could strongly remove pyrene in seawater, (2) with the increase of pyreneconcentrations in seawater, weever could be induced to metabolize and eliminatepyrene in vivo, (3) the higher pyrene concentration in seawater, the higher 1-pyrenol concentration in weever's bile, (4) pyrene concentration in weever's bile were much higher than that in seawater, weever's bile could strongly concentrate pyrene from seawater, (5) the concentration of 1-pyrenol and pyrene in weever'sbile had good correlation with pyrene concentration in seawater, which could beused as biomarkers to indicate pyrene concentration in seawater.