Structure and function of the seminal vesicle in male Bostrichthys sinensis
- 海洋环境－已发表论文 
应用组织学、透射电镜及酶联免疫吸附分析法研究了雄性中华乌塘鳢贮精囊的形态结构 ,并探讨其功能。结果表明 ,贮精囊是一对精巢的附属腺体 ,结缔组织隔膜将贮精囊分隔成为许多小室腔。被膜和隔膜中含有平滑肌纤维、毛细血管、成纤维和纤维细胞以及间质细胞。隔膜上排列单层上皮细胞 ,呈柱形、立方形或扁平形 ,生殖季节粗面内质网、管状嵴线粒体和高尔基复合体发达 ,上皮细胞顶部聚集许多无膜包裹的分泌颗粒 ,分泌后细胞器退化 ,胞质中出现大量的大空泡。成纤维细胞具有合成分泌胶原蛋白的结构特征。贮精囊中的间质细胞与精巢中的Leydig型间质细胞形态特征相似。在生殖高峰期不论贮精囊的近端、中央或远端均先后贮存大量的精子 ,混合在分泌物中 ,贮精囊不同小室的上皮细胞发育并不同步。精液加贮精囊液的实验表明 ,贮精囊液有助于增强精子活率和延长精子寿命 ,并能促进受精率的提高。贮精囊纤维丝状分泌物呈深紫红色的PAS阳性反应 ,提示分泌物为粘多糖蛋白 ,贮精囊液含有 17α羟孕酮、PGE2 和PGF2α,能体外诱发雌亲鱼产卵 ,具有性外激素的重要作用The morphological structure and function of the seminal vesicles in male Bostrichthys sinensis were studied by means of histology, transmission electron microscope and Enzyme-linked Immunosorbent assay. The results showed that the seminal vesicles, enclosed by capsula, were a pair of accessory glands to the testes. The seminal vesicle was divided into many locule lumina by the connective tissue partition. Both capsula and partition consisted of smooth muscle fibers, blood capillaries, fibroblasts, fibrocytes and interstitial cells. Single epithelial cells, which were columnar, cuboidal or squamous in shape, were arranged along the partition. During the spawning season, rough endoplasmic reticulum, tubular cristae mitochondria and Golgi complex were well developed; many secretory granules without membranes accumulated to the apical portion of the epithelial cell. The cell organelles degenerated and large vacuoles appeared after the secretory granules were released. The sphincter muscles were not found from the outlet of the seminal vesicles to the sperm ducts. Fibroblasts were characterized by synthesizing and secreting collagen. The morphology of interstitial cells in the seminal vesicle was similar to that of Leydig cells in the testes. At the peak period of spawning, a large number of spermatozoa, which were mixed with the secretion, appeared in proximal, middle and distal parts of the seminal vesicles. The epithelial cells inside the different locules of the seminal vesicle developed asynchronously. The results showed that sperm added to the seminal vesicle fluid could increase sperm motility, prolong sperm longevity, and improve the fertilization rates of spermatozoa. Fiber-like secretion of the seminal vesicles showed PAS positive reaction in dark purple-red colour, suggesting that the secretion should be mucopolysaccharide-mucin. The seminal vesicle fluid contained 17αOH-P, PGE 2 and PGF 2αwhich could induce female broodfish to spawn, indicating this fluid functions as a sex pheromone .