Molecular Cloning and Construction of Plant Expression Vector of Farnesyl Phosphate Synthase Gene from Menthor spicata L
- 海洋环境－已发表论文 
以留兰香(MenthaspicataL)为材料,利用RT PCR方法克隆法呢基焦磷酸合酶基因(fps)的cDNA,核酸序列分析表明,该基因的编码区长1050bp,编码349个氨基酸.进一步将fps基因插入植物表达载体BinAR,酶切鉴定及测序结果都证明fps的植物双元表达载体构建成功,为fps基因在烟草中的异源表达奠定了基础.Farnesyl phosphate synthase is a key enzyme in the biosynthesis pathway of sequiterpenoids, which play an important role in tobacco pathogen-resistance response. By RT-PCR technology, the cDNA of fps gene of Menthor spicata L was cloned and sequenced. The result demonstrated that the sequence of coding region was 1 050 bp,encodes a protein of 349 amino acid. In order to transfer fps gene into tobacco, plant expressing vector pBinARfps was constructed .