Show simple item record

dc.contributor.author苗利光
dc.contributor.author王克坚
dc.contributor.author陈立志
dc.contributor.author刘晓颖
dc.contributor.author徐敏
dc.contributor.author刘艳环
dc.date.accessioned2017-11-14T01:24:21Z
dc.date.available2017-11-14T01:24:21Z
dc.date.issued2002-11-30
dc.identifier.citation中国兽医学报,2002,(06):46-49
dc.identifier.issn1005-4545
dc.identifier.otherZSYX200206017
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/141484
dc.description.abstract用 PCR从腐蹄病 C型节瘤拟杆菌扩增出具有免疫保护性抗原 0 .85 kb纤毛蛋白基因 (pili基因 ) ,并构建了该基因的表达载体。转化宿主细胞 PAK/2 pfs,在营养肉汤中进行 pili基因的表达。培养 18~ 2 4h后 ,收集培养液上清 ,加入 0 .1mol/L Mg Cl2 ,离心提取重组纤毛蛋白。用羊抗兔 C型节瘤拟杆菌抗血清与提取的纤毛蛋白进行对流免疫电泳试验 ,结果表达的重组纤毛蛋白有特异性 ;用 SDS- PAGE和 Western- blot证明表达的蛋白是 C型节瘤拟杆菌纤毛蛋白。
dc.description.abstractThe pili gene that dominates the main protective immunogen was amplified and cloned from D.nodosus serorype C by PCR.An expression plasmid was constructed by cloning the pili gene into PME290.The plasmid harbouring the pili sequence was designated PME290-pili.The PME290-pili was transformed into the host competent cell PAK/2pfs and the recombinant pili was expressed in the supernatant of the cultures of the transformant cell PAK/2pfs.The recombinant pili was purified by MgCl 2 from the supernatant of the culture of the transformed PAK/2pfs.The specific reacion of the recombinant pili and antiserum of D.nodosus serotype C pili was demonstrated by cross electrophoresis.The recombinant pili was expressed at high level in PAK/2pfs.
dc.description.sponsorship国家科技部研究所技术开发研究专项基金项目〔国科财字 (1999) 5 92号〕
dc.language.isozh_CN
dc.subject腐蹄病
dc.subjectC型节瘤拟杆菌
dc.subject纤毛蛋白基因
dc.subject表达
dc.subjectfootrot
dc.subjectD.nodosus serotype C
dc.subjectfimbrial subunit gene(pili)
dc.subjectexpression
dc.title腐蹄病C型节瘤拟杆菌纤毛蛋白基因的克隆与表达
dc.title.alternativeCloning and Expression of D.nodosus Serotype C Pili Gene in Pseudomonas
dc.typeArticle


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record