Observations of the Primordial Germ Cells in Embryonic Blood and in Vivo DNA Transfer into the Primordial Germ Cells in Sheldrake
- 海洋环境－已发表论文 
抽取了麻鸭和骡鸭胚胎发育第 14～ 2 0期的血液 ,制作血涂片 ,PAS染色 .观察血液中的原生殖细胞的含量 ,并对各个时期血液中原生殖细胞的含量进行了统计 ,最高含量都出现在第 16期 ,分别为 8.975 2 %和 2 .1871% .用InvivoGeneDeliverySystem和 pEGFP N对PGCs进行了外源DNA的体内转染 .结果在 6只胚胎中的 3只中发现GFP报告基因的表达The amount of the primordial germ cells in embryonic blood from stages 14 through stages 20 in the Sheldrake's and mule-duck's embryos were counted by PAS reaction. The results showed that the highest concentration of primordial germ cells was at stages 16 both in Sheldrake and mule-duck, and the concentration was 8.975 2% for Sheldrake and 2.187 1% for mule-duck. Meanwhile, GFP report gene transfer into Sheldrake's blood PGCs in vivo was processed. The results showed that the expressions of GFP were found in 3 embryos.