Show simple item record

dc.contributor.author蒙子宁
dc.contributor.author庄志猛
dc.contributor.author金显仕
dc.contributor.author唐启升
dc.contributor.author苏永全
dc.date.accessioned2017-11-14T01:22:25Z
dc.date.available2017-11-14T01:22:25Z
dc.date.issued2003-05-25
dc.identifier.citation生物多样性,2003,(03):22-28
dc.identifier.issn1005-0094
dc.identifier.otherSWDY200303002
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/140470
dc.description.abstract小黄鱼 (Pseudosciaenapolyactis)是我国近海重要经济鱼类之一。本文分析了采自黄海和东海 5个海区共计4 8个个体小黄鱼的随机扩增DNA多态性 (RAPD)。从 4 0个 10bp引物中选取 2 0个用于群体遗传多样性分析 ,共检测出 14 5个位点 ,其中 132个 (91.0 3% )显多态性。用Shannon多样性指数量化的平均遗传多态度为 1.93(1.5 0~ 2 .4 4 ) ,群体内和群体间的遗传变异比例分别为 6 9%和 31% ;群体间的平均遗传相似度和遗传距离分别为 0 .9139和 0 .0 86 1。用非加权配对算数平均法 (UPGMA)聚类分析的结果表明 ,所分析的 5个群体可分为 3个地理群系 ,从分子水平上支持了过去有关学者把黄海和东海的小黄鱼划分为北、中、南 3个地理群系的观点
dc.description.abstractPseudosciaena polyactis is a commercially important fish species which is widely distributed in the Bohai Sea, the Yellow Sea and the northern part of the East China Sea. Forty eight individuals of P. polyactis from five sampling areas in the Yellow Sea and the East China Sea were analyzed by random amplified polymorphic DNA (RAPD) markers to determine the genetic variation among and within the stocks. A total of 145 loci were amplified using 20 random primers, of which 132 loci (91.03%) were polymorphic. Genetic diversity quantified by Shannon index varied from 1.50 to 2.44 with an average of 1.93. Partition of genetic variation indicated that 69% was distributed within stocks and 31% among stocks. The average genetic similarity and genetic distance were 0.9139 and 0.0861, respectively. Cluster analysis by UPGMA indicated that these five stocks might be divided into three groups. Results of RAPD analysis suggested extensive genetic diversity exists in this species and the genetic divergence among stocks is relatively high. The UPGMA dendrogram showed that there existed three geographic populations of P. polyactis in the Yellow Sea and the East China Sea, which supports previous conclusions based on morphological and ecological methods.
dc.description.sponsorship国家重点基础研究专项资助项目 (G19990 43 7)
dc.language.isozh_CN
dc.subject小黄鱼
dc.subject遗传变异
dc.subject地理群系
dc.subjectRAPD
dc.subjectPseudosciaena polyactis , genetic variation, geographic population, RAPD
dc.title黄海和东海小黄鱼遗传多样性的RAPD分析
dc.title.alternativeGenetic diversity in small yellow croaker (Pseudosciaena polyactis) by RAPD analysis
dc.typeArticle


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record