Genetic diversity in small yellow croaker (Pseudosciaena polyactis) by RAPD analysis
- 海洋环境－已发表论文 
小黄鱼 (Pseudosciaenapolyactis)是我国近海重要经济鱼类之一。本文分析了采自黄海和东海 5个海区共计4 8个个体小黄鱼的随机扩增DNA多态性 (RAPD)。从 4 0个 10bp引物中选取 2 0个用于群体遗传多样性分析 ,共检测出 14 5个位点 ,其中 132个 (91.0 3% )显多态性。用Shannon多样性指数量化的平均遗传多态度为 1.93(1.5 0～ 2 .4 4 ) ,群体内和群体间的遗传变异比例分别为 6 9%和 31% ;群体间的平均遗传相似度和遗传距离分别为 0 .9139和 0 .0 86 1。用非加权配对算数平均法 (UPGMA)聚类分析的结果表明 ,所分析的 5个群体可分为 3个地理群系 ,从分子水平上支持了过去有关学者把黄海和东海的小黄鱼划分为北、中、南 3个地理群系的观点Pseudosciaena polyactis is a commercially important fish species which is widely distributed in the Bohai Sea, the Yellow Sea and the northern part of the East China Sea. Forty eight individuals of P. polyactis from five sampling areas in the Yellow Sea and the East China Sea were analyzed by random amplified polymorphic DNA (RAPD) markers to determine the genetic variation among and within the stocks. A total of 145 loci were amplified using 20 random primers, of which 132 loci (91.03%) were polymorphic. Genetic diversity quantified by Shannon index varied from 1.50 to 2.44 with an average of 1.93. Partition of genetic variation indicated that 69% was distributed within stocks and 31% among stocks. The average genetic similarity and genetic distance were 0.9139 and 0.0861, respectively. Cluster analysis by UPGMA indicated that these five stocks might be divided into three groups. Results of RAPD analysis suggested extensive genetic diversity exists in this species and the genetic divergence among stocks is relatively high. The UPGMA dendrogram showed that there existed three geographic populations of P. polyactis in the Yellow Sea and the East China Sea, which supports previous conclusions based on morphological and ecological methods.