Immunostimulated blue algae as feed additive for Anguilla japonica black fry
- 海洋环境－已发表论文 
免疫增强蓝藻是把胸腺素α_1(Tα_1)基因转移到蓝藻——聚球藻PCC 7942(Synechococcus sp．PCC7942)染色体上后获得稳定表达的转基因蓝藻,经小鼠实验初步表明其具有生物学活性并且口服有效。通过采用该免疫增强蓝藻饲喂日本鳗鲡(Anguillajaponica)黑仔,并采集分离了一种鳗鱼养殖流行性发病弧菌,对饲喂了免疫增强蓝藻的黑仔鳗进行多次感染试验,结果表明：饲喂添加免疫增强蓝藻的饲料后,黑仔鳗各检测组织器官Tα_1含量显著提高；免疫增强蓝藻提高了试验鱼的免疫能力,增强了试验鱼的抗逆、抗病菌感染能力,并具有一定的促生长作用。免疫增强蓝藻作为鱼类饲料添加剂适口性好,适宜添加量为0．5～1．0 g·kg~(-1)。The immunostimulated blue algae is the transgenic Synechococcus sp. PCC7942 in which human thymosin-alpll (Tα_1) gene had been expressed effectively. It has been confirmed that the immunocompetence in mice can be improved by oral administration of immunostimulated Synechococcus sp. PCC7942. The immunostimulated Synechococcus sp. was fed to Anguilla japonica as feed additive in this experiment. And the black fry was infected with epidemic pathogenic Vibrio sp. which had been isolated from the diseased A. japonica in the laboratory. Ta, contents in the measured tissues and organs of the black fry increased after fed with immunostimulated Synechococcus sp. The immunocompetence in A. japonica could be improved by oral administration of immunostimulated Synechococcus sp.. Resistances to bacterial pathogens such as Vibrio sp. and to environmental stress could be increased by fed with this immunostimulated blue algae, and the effects of the immunostimulated blue algae facilitated on growth of the black fry have been also observed in the experiment. The immunostimulated blue algae was the feasible aquatic feed additive and its dosages was about 0.5 to 1.0 gram per kilogram formulated feed.