| dc.contributor.author | 陈子珺 | |
| dc.contributor.author | 王三英 | |
| dc.contributor.author | 陈晋安 | |
| dc.contributor.author | 彭宣宪 | |
| dc.date.accessioned | 2012-11-14T02:53:54Z | |
| dc.date.available | 2012-11-14T02:53:54Z | |
| dc.date.issued | 2002-09 | |
| dc.identifier.citation | 实验生物学报,2002,35(3):179-183 | zh_CN |
| dc.identifier.issn | 1674-2788 | |
| dc.identifier.uri | https://dspace.xmu.edu.cn/handle/2288/13886 | |
| dc.description | 通讯作者：彭宣宪 | zh_CN |
| dc.description.abstract | [中文摘要]肺癌蛋白质组的研究,有助于阐明其发病机制并对肺癌的防治有益。本文从研究人肺癌细胞热休克蛋白的表达情况出发,通过比较37℃、42℃和45℃培养条件下的人肺癌细胞A549总蛋白质的双向电泳图谱,获得3个温度敏感的差异蛋白点,依次命名为P1、P2、P3。对差异蛋白进行MALDI-TOF-MS分析和采用SWISS-PROT数据库中的Peptident软件检索后,初步鉴定P1与2种醛酮还原酶家族成员相匹配,P2可能为一种新蛋白,P3为锌指蛋白11A。[英文文摘]The study on proteome of human cancer is helpful to explain its pathogenesis and make good effect on its prevention and cure. We compared the 2-DE maps of whole proteins of human lung cancer line A549 at 37℃ , 42℃ and 45℃ for the purpose of studying the expression of its heat shock proteins. Three temperature-sensitive differential spots were obtained and named as P1,P2,P3, respectively. Analyzed by MALDI-TOF-MS and Peptident software searched in the SWISS-PROT database, the three differential proteins were elementarily identified. P; matches with two proteins belong to the Aldo-keto reductase family, P2 may be a new protein and P3 is Zinc finger protein 11A. | zh_CN |
| dc.description.sponsorship | 国家自然科学基金资助(30080029) | zh_CN |
| dc.language.iso | zh | zh_CN |
| dc.publisher | 《实验生物学报》编辑部 | zh_CN |
| dc.subject | 双向电泳 | zh_CN |
| dc.subject | 肽质量指纹谱 | zh_CN |
| dc.subject | 基质辅助激光解吸/飞行时间质谱 | zh_CN |
| dc.subject | 人肺癌细胞 | zh_CN |
| dc.subject | 热休克蛋白 | zh_CN |
| dc.subject | two-dimensional electrophoresis | zh_CN |
| dc.subject | PMF. MALDI-TOF mass spectrometry | zh_CN |
| dc.subject | human lung cancer cell； heat shock protein | zh_CN |
| dc.title | 温度对人肺癌细胞A549蛋白质表达的影响 | zh_CN |
| dc.title.alternative | THE INFLUENCE OF TEMPERATURE ON THE PROTEIN EXPRESSION OF HUMAN LUNG CANCER CELL LINE A549 | zh_CN |
| dc.type | Article | zh_CN |
