CYTOLOGICAL OBSERVATION OF ANTHER ABORTION AND STARCH DISTRIBUTION OF A CYTOPLASM MALE STERILE PEPPER (Capsicum annum L.)
- 生命科学－已发表论文 
[中文文摘]用PAS反应对辣椒细胞质雄性不育系8214A和保持系8214B花药中的淀粉粒分布进行研究。在减数分裂前,保持系花药与不育系花药的结构和淀粉粒分布相似。保持系花药减数分裂后,药壁绒毡层细胞开始液泡化并体积增大,在药隔薄壁细胞中积累了许多较小的淀粉粒;在小孢子晚期,绒毡层细胞退化,在药隔薄壁细胞中淀粉粒体积增大;在二胞花粉时期,随着花粉大液泡的消失花粉中出现淀粉粒;花粉成熟时,其细胞质中积累了丰富的淀粉粒。不育系花药减数分裂后,由于药室腔的空间不能扩大,四分体被挤压在一起,最终四分体小孢子败育。不育花药的维管组织发育正常,但较多的淀粉粒积累在药隔薄壁细胞中。该种辣椒雄性不育系中,花粉的败育发生在四分体时期。绒毡层细胞结构异常可能影响糖类物质向药室的正常转运。该种辣椒雄性不育系的绒毡层异常与花粉败育有关。[英文文摘] A cytoplasm male sterile pepper(Capsicum annum L.)was examined using cytochemical method to study its pollen abortion. Thick sections of both anthers of male sterility line 8214A and its maintainer 8214B at different stages were stained using Periodic Acid-Schiff′s (PAS) reaction to detect starch distribution. Anther structure and starch distribution in both anthers of male sterility and maintainer line were similar before the meiosis of microspore mother cells. After meiosis,the size of tapetal cells of fertile anthers of maintainer line increased and became high vacuolation. Abundant small starches appeared in the connective cells from tetrad stage to early stage of microspore development. At the late stage of microspore,the tapetal cells began to degenerate and the starches in the connective cells became large. Bi-cellular pollen synthesized starches after the large vacuole of vegetative cell disappeared,and abundant starches were stored in the mature pollen. In the anthers of male sterile line,meiosis of microspore mother could occurred and the tetrads could be formed in the locule,but the tetrads were extruded together because the locule could not enlarge its space. Finally,the tetrad microspores degenerated. The development of vascular tissue of the sterile anthers was normal and abundant starches were stored in the connective cells,which suggested that the function of plant transporting polysaccharide into anther was normal but tapetum could not transport the polysaccharide into locule. According to our result,the pollen abortion occurred in the tetrad stage and the abnormal development of tapetal cells might be the reason which induced tetrad microspore abortion in this male sterile pepper.