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dc.contributor.author伍成厚
dc.contributor.author赵玉辉
dc.contributor.author杨延红
dc.contributor.author田惠桥
dc.date.accessioned2012-10-22T01:11:13Z
dc.date.available2012-10-22T01:11:13Z
dc.date.issued2012-08
dc.identifier.citation热带亚热带植物学报,2012,20(4):393-398zh_CN
dc.identifier.issn1005-3395
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/13805
dc.description作者简介:伍成厚, 男, 博士后, 园林植物生殖生物学研究方向。E-mail: wuchenghou2000@yahoo.com.cn 通讯作者 Corresponding author. E-mail: hqtian@jingxian.xmu.edu.cnzh_CN
dc.description.abstract[中文文摘]五唇兰(Doritis pulcherrima)具二胞花粉,授粉后1 d即有花粉开始萌发,授粉后5 d开始有生殖细胞完成有丝分裂形成一对精细胞。通过人工授粉使花粉管在子房内发育,再利用花粉管直接爆破,成功分离出五唇兰的精细胞。成对的2个精细胞在直径大小、荧光强弱上均显示出较大差异,预示2个精细胞具有不同的前途。[英文文摘]The mature pollen of Doritis pulcherrima is two-celled.The pollen began to germinate after pollinated one day.After pollinated five days,the division of generative cell occurred in pollen tube and formed a pair of sperm cells.The pollen tubes were induced to development in the ovary after artificial pollination and sperm cells were isolated from the tubes by immediately blowing up in a broken solution containing 5%–12% mannitol.The two sperm cells isolated were dimorphism,one was big and the other small,and the fluorescent intensity was distinctly different.It suggested that both sperm cells would have different futures.zh_CN
dc.description.sponsorship广东省科技计划项目(2007B020801007);广州市科技计划项目(11A63030233)资助zh_CN
dc.language.isozhzh_CN
dc.publisher《热带亚热带植物学报》编辑部zh_CN
dc.subject五唇兰zh_CN
dc.subject生殖细胞zh_CN
dc.subject精细胞zh_CN
dc.subject花粉管爆破zh_CN
dc.subject精细胞分离zh_CN
dc.subjectDoritis pulcherrimazh_CN
dc.subjectGenerative cellzh_CN
dc.subjectSperm cellzh_CN
dc.subjectPollen tube blow upzh_CN
dc.subjectSperm cell isolationzh_CN
dc.title五唇兰精细胞的分离zh_CN
dc.title.alternativeIsolation of Sperm Cells in Doritis pulcherrimazh_CN
dc.typeArticlezh_CN


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