九龙江口海岸带沉积物甲烷相关古菌群落结构及多样性研究

Abstract

[中文文摘]目的]针对河口环境的甲烷产生相关微生物展开调查,了解其多样性及群落结构组成、变化。[方法]采用克隆文库技术,分析九龙江河口沉积物样品,调查甲烷产生相关微生物的多样性及分布情况。[结果]九龙江沉积物含有清晰的甲烷硫酸盐过渡区。克隆文库分析结果显示主要是Methanosaeta,Methanomicrobiales和Methanomsarcinales/ANME。Methanomsarcinales/ANME是主要类群(41%),且以甲烷氧化菌ANME-2a为主。Methanosaeta和Methanomicrobiales分别占27%和28%。[结论]在九龙江河口沉积物中,乙酸利用型和氢气利用型甲烷产生是主要的甲烷产生途径。[英文文摘][Objective] To investigate the diversity and distribution of methanogen in the sediments of Jiulong River estuary.[Method] Based on clone library analysis,the diversity and distribution of methanogen communities in sediments of Jiulong River estuary were investigated.[Result] The majority of obtained sequences in clone library were assigned mainly to Methanosaeta,Methanomicrobiales and Methanomsarcinales/ANME.The sequences of Methanomsarcinales/ANME were the most dominant group(41% of total sequences) and composed largely of ANME-2a． In ad¬dition，Methanosaeta and Methanomicrobiales accounted for 27% and 28%of all sequences．［Conclusion］ Acetoclastic and hydrogenotrophicmethanogenesis were the main methanogenic pathway in Jiulong River estuary sediments．