Now showing items 955-974 of 2316

    • T_H细胞在记忆性CTL介导的肿瘤抑制中的作用研究 

      高丰光; Germain J. P. Fernando(University of Queensland,Australia); 刘文军(University of Queensland,Australia) (中华微生物学和免疫学杂志, 2005)
      观察记忆性CTL的抑瘤作用是否需要抗原特异性TH 细胞的辅助。方法 表达OVAT细胞表位SIINFEKL特异性TCR的T细胞转输RAG- - 小鼠,经相应T细胞表位多肽刺激后产生记忆性CTL ,将此记忆性CTL转输已产生特异性和非特异性TH 细胞的C5 7BL 6小鼠体内并接种肿瘤细胞EG7。结果 经T细胞表位多肽免疫后,SIINFEKL特异性TCRT细胞成功在RAG- - 小鼠体内增殖并分化为记忆性CTL ;抗原特异性TH ...
    • Tailoring 3,3 '-Dihydroxyisorenieratene to Hydroxystilbene: Finding a Resveratrol Analogue with Increased Antiproliferation Activity and Cell Selectivity 

      Kang, Yan-Fei; Yan, Wen-Jing; Zhou, Ting-Wen; Dai, Fang; Li, Xiu-Zhuang; Bao, Xia-Zhen; Du, Yu-Ting; Yuan, Cui-Hong; Wang, Hai-Bo; Ren, Xiao-Rong; Liu, Qiang; Jin, Xiao-Ling; Zhou, Bo; Zhang, Jie; 张杰 (WILEY-V C H VERLAG GMBH, 2014-07-14)
      Four novel compounds were designed by "tailoring" 3,3'-dihydroxyisorenieratene (a natural carotenoid) based on an isoprene unit retention truncation strategy. Among them, the smallest molecule 1 (2,3,6,2', 3', 6'-hexamet ...
    • Targeting the Vasculature of Colorectal Carcinoma with a Fused Protein of (RGD)(3)-tTF 

      Huang, Zheng-jie; Zhao, Yilin; Luo, Wei-yuan; You, Jun; Li, Shui-wen; Yi, Wen-cheng; Wang, Sheng-yu; Yan, Jiang-hua; Luo, Qi; 黄正接; 赵一麟; 尤俊; 颜江华; 罗琪 (HINDAWI PUBLISHING CORPORATION, 2013)
      Purpose. Truncated tissue factor (tTF) fusion protein targeting tumor vasculature can induce tumor vascular thrombosis and necrosis. Here, we generated (RGD)(3)-tTF in which three arginine-glycine-aspartic (RGD) targeting ...
    • Tat competes with HEXIM1 to increase the active pool of P-TEFb for HIV-1 transcription 

      Barboric, M; Yik, JHN; Czudnochowski, N; Yang, ZY; Chen, RC; Contreras, X; Geyer, M; Peterlin, BM; Zhou, Q; 陈瑞川 (2007-03)
      Human immunodeficiency virus type 1 (HIV-1) transcriptional transactivator (Tat) recruits the positive transcription elongation factor b (P-TEFb) to the viral promoter. Consisting of cyclin dependent kinase 9 (Cdk9) and ...
    • Tat peptide-decorated gelatin-siloxane nanoparticles for delivery of CGRP transgene in treatment of cerebral vasospasm 

      Tian, Xin-Hua; Wang, Zhi-Gang; Meng, Han; Wang, Yu-Hua; Feng, Wei; Wei, Feng; Huang, Zhi-Chun; Lin, Xiao-Ning; Ren, Lei; 田新华; 魏峰; 林晓宁; 任磊 (DOVE MEDICAL PRESS LTD, 2013)
      Background: Gene transfer using a nanoparticle vector is a promising new approach for the safe delivery of therapeutic genes in human disease. The Tat peptide-decorated gelatin-siloxane (Tat-GS) nanoparticle has been ...
    • Tat peptide-decorated gelatin-siloxane nanoparticles for delivery of CGRP transgene in treatment of cerebral vasospasm (vol 8, pg 865, 2013) 

      Tian, Xin-Hua; Wang, Zhi-Gang; Meng, Han; Wang, Yu-Hua; Feng, Wei; Wei, Feng; Huang, Zhi-Chun; Lin, Xiao-Ning; Ren, Lei; 田新华; 丰伟; 魏峰; 林晓宁; 任磊 (DOVE MEDICAL PRESS LTD, 2013)
    • Tat-TTA1-PEG共修饰明胶-硅氧烷纳米粒跨血脑屏障及对胶质瘤的靶向性研究 

      张龙; 胡洋; 林晓宁; 魏峰; 丰伟; 田新华 (2014-11-28)
      目的观察经TAT多肽,适配体TTA1,聚乙二醇(PEg)联合修饰的新型纳米载体明胶硅氧烷纳米粒子(gElATIn-SIlOXAnE nAnOPArTIClES,gS nPS)跨血脑屏障及靶向到达胶质瘤的能力。方法通过两步溶胶-凝胶法合成gS nPS,然后在其表面依次修饰上PEg、TTA1、TAT,并以荧光染料Cy5.5-nHS标记。建立胶质瘤裸鼠原位模型20~25只,分为空白对照组,gS nPS组,PEg-gS nPS组,TTA1-PEg-gS ...
    • TFF1在功能性消化不良和慢性胃炎中的表达 

      王琳; 卢雅丕; 任建林; 施华秀 (2003-08-20)
      目的 测定三叶因子1(trefoil factor,TFF1)在正常、功能性消化不良、慢性胃炎胃粘膜中的表达情况,探讨TFF1与功能性消化不良和慢性胃炎的关系。方法 应用免疫组化方法测定正常人和功能性消化不良、慢性胃炎患者胃窦黏膜中TFF1的表达,通过图像分析软件分析其阳性信号平均光密度值。结果 慢性胃炎组TFF1的表达明显高于正常组和功能性消化不良组,而正常组和功能性消化不良组中表达均较弱,二者统计学上无差异。结论 ...
    • TFF1在正常及损伤胃黏膜中的表达改变 

      任建林; 卢雅丕; 王琳; 陈建民; 施华秀; 叶震世; 吴艳环; 钟燕; 林逊汀; 林辉; 潘金水; 罗金燕 (2003-11-15)
      目的:通过观察 TFF1在人消化性溃疡及阿司匹林诱导的兔胃溃疡中的表达,探讨 TFF1在胃黏膜保护中的作用.方法:采用免疫组化方法测定正常及消化性溃疡患者、正常及阿司匹林诱导的兔胃溃疡模型中胃黏膜 TFF1的表达,通过图像分析软件分析其阳性信号平均光密度值了解其表达情况.结果:(1)消化性溃疡患者胃黏膜中 TFF1的表达高于正常胃黏膜(P<0.01);其中,多发/复合性溃疡表达高于单发消化性溃疡(P<0.05).(2)阿司匹林诱导的兔胃 ...
    • TFF3 mediated induction of VEGF via hypoxia in human gastric cancer SGC-7901 cells 

      Guleng, Bayasi; Han, Jia; Yang, Jin-Qiu; Huang, Qing-Wen; Huang, Jian-Kun; Yang, Xiao-Ning; Liu, Jing-Jing; Ren, Jian-Lin; 任建林 (SPRINGER, 2012-04)
      Increasing evidence indicates that in gastric epithelial cells, induction of TFF3 by hypoxia is mediated by HIF-1. Since VEGF is one of the most important angiogenic factors on cancer progression, we have started to ...
    • TGF-beta 1 induces autophagy and promotes apoptosis in renal tubular epithelial cells 

      Xu, Yanfang; Yang, Shuyu; Huang, Jiyi; Ruan, Shiwei; Zheng, Zhang; Lin, Jiumao; 黄继义 (INT J MOL MED, 2012-05)
      Transforming growth factor-beta 1 (TGF-beta 1) is a multifunctional cytokine that regulates cell growth, differentiation, apoptosis and autophagy in various cell types. It has been shown that TGE-beta 1-driven autophagy ...
    • TGF-beta of lung cancer microenvironment upregulates B7H1 and GITRL expression in dendritic cells and is associated with regulatory T cell generation 

      Ni, Xiao Yan; Sui, Hua Xiu; Liu, Yao; Ke, Shi Zhong; Wang, Yi Nan; Gao, Feng Guang; 高丰光 (SPANDIDOS PUBL LTD, 2012-08)
      The effects of TGF-beta on dendritic cells (DCs) on the tumor microenvironment are not well understood. We report, here, the establishment of an in vitro lung cancer microenvironment by co-incubation of seminaphtharho-dafluor ...
    • TGF-beta1诱导大鼠肝星状细胞系HSC-T6活化及上皮间质转换 

      王丽惠; 程变巧; 朱琪; 林伟国 (2017)
      目的探讨转化生长因子-beta1(TGF-beta1)在大鼠肝星状细胞系(HSC-T6)活化及上皮间质转换(EMT)中的作用。方法体外培养HSC; -T6,用MTT法筛选TGF-beta1对HSC-T6作用的最佳浓度;用10 mug/L TGF-beta1处理HSC-T6 24; h,相差倒置显微镜下观察细胞形态改变,免疫荧光染色法检测细胞骨架结构F-actin蛋白的表达,; RT-qPCR法检测肌动蛋白alpha-SMA及代表上皮间质 ...
    • TGF-β1诱导大鼠肝星状细胞系HSC-T6活化及上皮间质转换 

      王丽惠; 程变巧; 朱琪; 林伟国 (2017)
      目的探讨转化生长因子-β1(TGF-β1)在大鼠肝星状细胞系(HSC-T6)活化及上皮间质转换(EMT)中的作用。方法体外培养HSC-T6,用MTT法筛选TGF-β1对HSC-T6作用的最佳浓度;用10μg/L TGF-β1处理HSC-T624h,相差倒置显微镜下观察细胞形态改变,免疫荧光染色法检测细胞骨架结构F-actin蛋白的表达,RT-qPCR法检测肌动蛋白α-SMA及代表上皮间质转换的神经黏附素(N-cadherin)、波形蛋白 ...
    • TGF-β及EGF信号对溶血磷脂酸诱导口腔鳞癌细胞增殖的影响 

      周小琼; 邓小玲; 傅玉才; 许铭炎 (2014-1-30)
      目的:探讨转化生长因子(Tgf)及表皮生长因子(Egf)信号对溶血磷脂酸(lPA)诱导口腔鳞癌细胞增殖的影响。方法:口腔鳞癌SAS细胞接种于96孔板中,以含不同浓度(0、1、5和10μMOl/l)lPA的无血清dMEM分别处理细胞24和48 H后采用CCk-8法检测细胞增殖;免疫印迹检测10μMOl/l lPA分别处理细胞0、0.5、2和4 H后对Tgf-β下游信号分子SMAd2的影响;应用不同浓度(10、20和50μg/Ml)的Tgf ...
    • The amino acid variation within the binding pocket 7 and 9 of HLA-DRB1 molecules are associated with primary Sjögren's syndrome 

      Renliang Huang; 黄仁良; Junping Yin; Yan Chen; Fengyuan Deng; Juan Chen; Xing Gao; Zuguo Liu; Xinhua Yu; Junfeng Zheng; 郑俊峰 (Elsevier Ltd., 2015-01-09)
      Primary Sjögren's syndrome (pSS) is associated with HLA-DRB1 loci, but the association of amino acid variations in the hypervariable region of the HLA-DR β1 chain with pSS is largely unknown. In this study, we aimed to ...
    • The association of blood pressure with survival rate and cardiovascular events in Chinese patients with type 2 diabetes 

      Wu, Shouling; Chi, Hongjie; Jin, Cheng; Huang, Zhengrong; Chen, Li; Wu, Yuntao; Xin, Aijun; Ruan, Chunyu; Li, Kuibao; Gao, Jingsheng; Cai, Jun; 黄峥嵘 (ELSEVIER IRELAND LTD, 2013-Oct 9)
    • The compensatory enrichment of sphingosine-1-phosphate harbored on glycated high-density lipoprotein restores endothelial protective function in type 2 diabetes mellitus 

      Tong, Xunliang; Lv, Pu; Mathew, Anna V.; Liu, Donghui; Niu, Chenguang; Wang, Yan; Ji, Liang; Li, Jizhao; Fu, Zhiwei; Pan, Bing; Pennathur, Subramaniam; Zheng, Lemin; Huang, Yining; 刘东辉; 王焱 (BIOMED CENTRAL LTD, 2014-04-21)
      Background: Glycation of high-density lipoprotein (HDL) decreases its ability to induce cyclooxygenase-2 (COX-2) expression and prostacyclin I-2 (PGI-2) release in endothelial cells. Whether lipid content of HDL, especially ...
    • The competition between two polarization states in two-dimensional random medium 

      Liu, H.; Liu, J. S.; Feng, B.; Zhang, Q. Q.; Lu, J. T.; Zhu, H.; 张其清 (2008)
      In a two-dimensional random medium, when the transverse magnetic (TM) polarization state and the transverse electric (TE) polarization state share the same population inversion, there is a competition in the population ...