Now showing items 402-421 of 2316

    • Fabrication of chitosan/hydroxylapatite composite rods with a layer-by-layer structure for fracture fixation 

      Pu, Xi-Ming; Sun, Zhen-Zhen; Hou, Zhen-Qing; Yang, Yun; Yao, Qing-Qing; Zhang, Qi-Qing; 张其清 (WILEY-BLACKWELL, 2012-07)
      A composite rod for fracture fixation using chitosan (CHI)/hydroxylapatite (HA) was prepared by means of in situ precipitation, which had a layer-by-layer structure, good mechanical properties, and cell compatibilities. ...
    • Fabrication of Cluster/Shell Fe3O4/Au Nanoparticles and Application in Protein Detection via a SERS Method 

      Zhou, X.; Xu, W. L.; Wang, Y.; Kuang, Q.; Shi, Y. F.; Zhong, L. B.; Zhang, Q. Q.; 张其清 (2010-11-25)
      We have successfully synthesized cluster/shell Fe3O4/Au nanoparticles using a pH-sensitive polyethyleneimine (PEI) linker. We propose that PEI binds gold seeds, prevents the formation of large aggregates, and changes the ...
    • Fabrication of composition-graded collagen/chitosan-polylactide scaffolds with gradient architecture and properties 

      Yin, Dengke; Wu, Hua; Liu, Chaoxu; Zhang, Jun; Zhou, Ting; Wu, Jingjing; Wan, Ying; 吴华; 张军 (Elsevier, 2014 Octob)
      Chitosan-polylactide (CH-PLA) copolymers with various polylactide percentages changing from around 14 to 40 wt% were synthesized. CH-PLAs were then blended with type-II collagen to fabricate layered collagen/CH-PLA scaffolds ...
    • Fabrication of core-shell microspheres using alginate and chitosan-polycaprolactone for controlled release of vascular endothelial growth factor 

      Wu, Hua; Liao, Chunyan; Jiao, Qianyi; Wang, Zheng; Cheng, Wenze; Wan, Ying; 吴华 (REACT FUNCT POLYM, 2012-07)
      Alginate microspheres loaded with vascular endothelial growth factor (VEGF) were prepared via an emulsification method using calcium chloride as a crosslinker. The microspheres with encapsulation efficiency of about 80% ...
    • FACILE SYNTHESES OF THREE AHP-TYPE BUILDING BLOCKS WITH COMPLEMENTARY REACTIVITIES 

      Chen,W; Huang,PQ; Ruan,YP; Zheng,X; 郑啸 (2009-04-01)
      Two protected 3-amino-6-hydroxy-2-piperidone derivatives (Ahp, 12 and 6), as well as a thioether analogue (7) were synthesized starting from L-glutamic acid in four, five, and six steps respectively. The Ahp derivatives ...
    • Factors Associated with Serological Cure and the Serofast State of HIV-Negative Patients with Primary, Secondary, Latent, and Tertiary Syphilis 

      Tong, Man-Li; Lin, Li-Rong; Liu, Gui-Li; Zhang, Hui-Lin; Zeng, Yan-Li; Zheng, Wei-Hong; Liu, Li-Li; Yang, Tian-Ci; 林丽蓉; 郑维红; 刘莉莉; 杨天赐 (PUBLIC LIBRARY SCIENCE, 2013-Jul 2)
      Background: Some syphilis patients remain in a serologically active state after the recommended therapy. We currently know too little about the characteristics of this serological response. Methods: We conducted a cohort ...
    • Failure mode and effects analysis using D numbers and grey relational projection method 

      Liu, Hu-Chen; You, Jian-Xin; Fan, Xiao-Jun; Lin, Qing-Lian; 樊孝俊 (PERGAMON-ELSEVIER SCIENCE LTD, 2014 AUG)
      Failure mode and effects analysis (FMEA) is a widely used risk assessment tool for defining, identifying and eliminating potential failures or problems in products, process, designs and services. Two critical issues of ...
    • FasL、抗人DR5单克隆抗体诱导肿瘤细胞凋亡的研究 

      宋玉国; 李文珠; 李迎; 陈彩霞; 庄国洪 (中国免疫学会;吉林省卫生厅, 2007)
      目的探讨FasL、Anti-DR5mAb对肿瘤细胞Hela、BGC823、MCF-7、L342、H9101、D6的杀伤作用及机制。方法采用RT-PCR、MTT比色法、电泳、DNA倍体分析、Westernblot等方法。结果H9101、Hela细胞株DR5mRNA水平有表达,D6细胞无表达;H9101、L342细胞株FasmRNA水平有表达,D6细胞无表达。H9101、L342细胞株对FasL、Anti-DR5mAb敏感并呈剂量依赖性;M ...
    • FasL基因构建 蛋白表达及其对胃癌细胞株BGC823和MGC803生长的影响 

      刘忠臣; 李文珠; 齐忠权; 丁志杰; 袁思波; 牛丽文; 庄国洪 (2009-09-30)
      目的:构建适于原核表达的重组蛋白FasL表达载体,并进行重组蛋白的表达纯化及抗肿瘤活性分析。方法:获得人FasL cDNA全序列,将其分段设计引物,通过重叠PCR获得FasL基因。构建pGEX-5X-1/FasL表达载体。转化大肠杆菌BL21(DE3),IPTG诱导表达,GST柱纯化。采用MTT比色法、流式细胞法检测融合蛋白对胃癌细胞的作用。结果:通过重叠PCR获得了编码正确氨基酸序列的目的基因。表达的目的蛋白表达量占菌体总蛋白的30% ...
    • Fas和DcR3在垂体腺瘤的表达及FasL与RGD-FasL诱导的细胞凋亡机制 

      陈陆馗; 康俊龙; 刘运生; 候永宏; 张俊卿; 田新华; 李文珠; 庄国洪 (2008-04-30)
      目的研究RGD-FasL诱导垂体腺瘤细胞株GH3、MMQ、AtT20所产生的细胞毒性效应,并探讨其机制。方法应用RT-PCR法和流式细胞仪检测肿瘤细胞上Fas、DcR3的表达。应用MTT测定法检测FasL、RGD-FasL对肿瘤细胞所产生的细胞毒性效应,并经琼脂糖凝胶电泳证实是否为该配体所诱导的凋亡。通过流式细胞仪PI染色法评价细胞周期的变化和凋亡分析。通过Western蛋白印迹法检测Caspase 8、Caspase 9、Caspase ...
    • Fas胞外区基因的构建、表达、纯化及多克隆抗体的制备 

      张佳锴; 孟庆宇; 程小峰; 刘瑞振; 庄国洪 (2010-02-20)
      目的构建Fas胞外区(eFas)基因的表达载体,表达纯化重组蛋白,进行多克隆抗体的制备,为进一步功能研究奠定基础。方法通过重叠PCR获得eFas基因的编码序列,构建pET-22b(+)/eFas表达载体,转化大肠杆菌Rosetta-gami,IPTG诱导表达,Ni-NTA柱亲和纯化,SDS-PAGE鉴定重组蛋白的纯度。将纯化的eFas融合蛋白免疫新西兰白兔制备多克隆抗体,通过ELISA方法检测多克隆抗体的效价。结果获得了eFas的编码序 ...
    • Feasibility of atlantoaxial pedicle screws' placement in children 6-8 years of age: a cadaveric and tomographic study 

      Lin, Bin; Xu, Yang; Guo, Zhi-Min; Liu, Hui; Chen, Zhi-Wen; He, Min-Chang; 林斌; 徐洋; 郭志民; 陈志文 (LIPPINCOTT WILLIAMS & WILKINS, 2013)
      The aims of this study were to evaluate the linear and angular dimensions of children's atlantoaxial pedicle using cadavers combined with axial computed tomography (CT) and to examine the feasibility of placing atlantoaxial ...
    • Fecal Microbiota Transplantation Induces HBeAg Clearance in Patients with Positive HBeAg after Long-Term Antiviral Therapy 

      Yan-Dan Ren; Zhen-Shi Ye; Liu-Zhu Yang; Li-Xin Jin; Wen-Jun Wei; Yong-Yue Deng; Xiao-Xiao Chen; Chuan-Xing Xiao; Xiao-Fang Yu; Hong-Zhi Xu; Li-Zhu Xu; Yun-Na Tang; Fei Zhou; Xue-Lian Wang; Mei-Ya Chen; Li-Gang Chen; Mei-Zhu Hong; Jian-Lin Ren; 任建林; Jin-Shui Pan; 潘金水 (John Wiley & Sons, Inc., 2016-12-27)
      【Abstract】For hepatitis B virus (HBV) e-antigen (HBeAg)-positive chronic hepatitis B (CHB)patients, HBeAg seroconversion is a prerequisite for a definite course of antiviral treatment. Unfortunately, for both entecavir ...
    • Ferritin light chain interacts with PEN-2 and affects gamma-secretase activity 

      Li, Xinxin; Liu, Yiqian; Zheng, Qiuyang; Yao, Guorui; Cheng, Peng; Bu, Guojun; Xu, Huaxi; Zhang, Yun-wu; 卜国军; 许华曦; 张云武 (ELSEVIER IRELAND LTD, 2013-Aug 2)
      Alzheimer's disease (AD) is primarily caused by overproduction/deposition of beta-amyloid (A beta) in the brain. Dysregulation of iron in the brain also contributes to AD. Although iron affects beta-amyloid precursor protein ...
    • Fe~(3+)/TiO_2光催化降解溴化乙锭的研究 

      黄菊; 向蓉; 刘晔; 李东辉 (2007-07-15)
      The possibility using anatase titanium dioxide as a photocatalyst for the degradation of EB was investigated in this article. Factors including light sources, pH, irradiation time and the usage of catalyst that influenced ...
    • Fibrin glue-assisted for the treatment of corneal perforations using glycerin-cryopreserved corneal tissue 

      Dong, Nuo; Li, Cheng; Chen, Wen-Sheng; Qin, Wen-Juan; Xue, Yu-Hua; Wu, Hu-Ping; 董诺 (IJO PRESS, 2014-02-18)
      AIM: To evaluate the outcomes and safety of lamellar keratoplasty (LK) assisted by fibrin glue in corneal perforations. METHODS: Six eyes of 6 patients affected by different corneal pathologies (2 posttraumatic corneal ...
    • Firing patterns of rat vestibulospinal neurons during quadrupedal standing on a pitching platform 

      Fujiwara, S.; Saito, T.; Tian, F.; Yamaguchi, T.; 田风 (J PHYSIOL SCI, 2006-10)
      The neuronal activity of vestibulospinal neurons projecting to the lumbar enlargement was recorded in conscious rats standing with four limbs on a pitching platform. The neurons were classified into 3 groups: up-neurons ...
    • Follow-up examination of 12 heart transplant recipients with cardiac CT 

      Duan Shaoyin; Yin Yongmei; Sui Tong; Cai Guoxiang; Liao Chongxian; 段少银; 廖崇先 (CLIN IMAG, 2012-NOV-D)
      Objective: The aims of this study were to observe the changes of a transplanted heart with cardiac computed tomography (CT) and to evaluate the clinical application of the examination. Methods: Cardiac CT was performed on ...
    • Formaldehyde suppresses neuronal apoptosis via inhibition of outward K+ currents in rat hippocampus 

      Liu, Pei; Guo, Jianli; Qi, Zhi; 戚智 (CAN J PHYSIOL PHARM, 2011-09)
      Formaldehyde (FA) is widely present in the environment, and is also a mammalian metabolite. However, its biological role has not been well understood. Here, we show that FA plays an anti-apoptotic role in cultured hippocampal ...
    • Formation of poly(L,D-lactide) spheres with controlled size by direct dialysis 

      Liu, M.; Zhou, Z. M.; Wang, X. F.; Xu, J.; Yang, K.; Cui, Q.; Chen, X.; Cao, M. Y.; Weng, J.; Zhang, Q. Q.; 张其清 (2007-09-10)
      In this work, we show that simple dialysis of a poly(DL-lactic acid) (PLA) solution against water resulted in the spontaneous formation of PLA spheres. We observed initial formation of particles with irregular shape and ...