Application of thiamine as a fluorogenic substrate in the determination of hydrogen peroxide based on the catalytic effect of hemin
- 化学化工－已发表论文 
A rapid and sensitive fluorimetric method has been developed for the determination of trace hydrogen peroxide (0 - 5.0 x 10(-6) mol/l) and, indirectly, glucose (3.0 x 10(-7) - 3.5 x 10(-5) mol/l), based on the catalytic effect of hemin on the thiamine, a new fluorogenic substrate, and hydrogen peroxide system at pH=8.0. Using the fixed time method, the detection limits of hydrogen peroxide and glucose are 3.0 x 10(-8) mol/l and 3.0 x 10(-7) mol/l, respectively. This method is simple, inexpensive and has been applied successfully to the determination of glucose in human sera with relative standard deviation of 2.4 - 3.1%.