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Performance of HRP enzyme electrode in 1,4-dioxane media

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1_4_二氧六环介质中辣根过氧化物酶电极的性能研究.pdf (368.7Kb)
Date
1996
Author
Chen, H
陈红
Wu, HH
吴辉煌
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  • 化学化工-已发表论文 [14469]
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Abstract
The horseradish peroxidase (HRP) electrode was made by crosslinkage method,and its electrochemical behaviour was studied in 1,4-dioxane media. It is shown that the immobilized HRP can maintain its activity in organic phase and the direct electron transfer occurs between HRP and the electrode substrate, hence the HRP electrode can catalyze the electrochemical reduction of H2O2 in the absence of mediators. When HRP and K4Fe(CN)(6) are comdified on the basis electrode,the performance of HRP electrode will be improved significantly since K4Fe(CN)6 acts as the electron transfer mediator in the redox process of enzyme. According to the kinetic parameters obtained in different conditions, the factors influencing the performance of the HRP electrode are disscused.
Citation
ACTA CHIMICA SINICA,1996,54(9):882-887
URI
https://dspace.xmu.edu.cn/handle/2288/13603

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