Performance of HRP enzyme electrode in 1,4-dioxane media

Abstract

The horseradish peroxidase (HRP) electrode was made by crosslinkage method,and its electrochemical behaviour was studied in 1,4-dioxane media. It is shown that the immobilized HRP can maintain its activity in organic phase and the direct electron transfer occurs between HRP and the electrode substrate, hence the HRP electrode can catalyze the electrochemical reduction of H2O2 in the absence of mediators. When HRP and K4Fe(CN)(6) are comdified on the basis electrode,the performance of HRP electrode will be improved significantly since K4Fe(CN)6 acts as the electron transfer mediator in the redox process of enzyme. According to the kinetic parameters obtained in different conditions, the factors influencing the performance of the HRP electrode are disscused.