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Determination of DNA by use of in situ photochemical fluorescence probe - Sodium 9,10-anthraquinone-2-sulfonate as a photochemical fluorescence probe

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现场光化学荧光探针法测定DNA_以9_10_蒽醌_2_磺酸钠为光化学荧光探针.pdf (127.2Kb)
Date
1996
Author
Li, WY
李文友
Xu, JG
许金钩
Guo, XQ
郭祥群
Zhu, QZ
朱庆枝
Zhao, YB
赵一兵
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  • 化学化工-已发表论文 [14469]
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Abstract
An in situ photochemical fluorescence probe method for the determination of DNA with sodium 9,10-anthraquinone-2-sulfonate (AQS) as a photochemical fluorescence probe was developed for the first time, It was based on the conversion of AQS into an intensively fluorescent product on irradiating with UV radiation. The photochemical reaction is decelerated by DNA, The determination can be carried out by measuring the fluorescence intensity at a fixed time, The calibration graph was linear in the range 0 similar to 80 ng/mL CT DNA(r=0.999 1), the limit of detection was 3.2 ng/mL CT DNA (n=9), The kinetic behaviour of the photochemical reaction and the effects of some experiment conditions were investigated and discussed in detail, The results of absorption spectra experiments and competitive binding experiments suggest that the interaction between AQS and DNA may be intercalative.
Citation
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,1996,17(11):1706-1707
URI
https://dspace.xmu.edu.cn/handle/2288/13590

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