Protein conformation in solution by three-dimensional fluorescence spectrometry
- 化学化工－已发表论文 
The conformations of bovine serum albumin (BSA) and egg albumin (EA) in solution and their conformation changes under different conditions were studied by using three-dimensional fluorescence spectrometry (TDFS) such as three-dimensional fluorescence (TDF) spectra and three-dimensional fluorescence polarization (TDFP) spectra with tryptophan residues in protein molecules as an intrinsic fluorescent probe. The results show that the microenvironment of tryptophan residues of protein molecules in various solutions can be directly indicated arid TDFS is an effective tool for studying protein conformation in solution. Meantime, some valuable results were obtained.