Non-enzymatic system for catalytic fluorometric determination of glucose oxidase
- 化学化工－已发表论文 
A novel non-enzymatic method for the determination of oxidases and their substrates was proposed, The principle was examined by fluorometric determination of glucose oxidase(GOD). Using EGTA(NH4)(2)Fe(SO4)(2) as modified Fenton reagent, H2O2 the product of GOD catalytic reaction was converted into hydroxyl radical, the later oxidized terephthalic acid, which was chosen from four fluorogenic indicator of hydroxyl radical, into fluorescent product GOD was thus measured according to the reaction rates. The method is simple, stable, cheap and at least as sensitive as its counterpart enzymatic method.